Jame C McCrae1, Noel Sharkey2, David J Webb3, A D Bastiaan Vliegenthart3, James W Dear3. 1. a Ashworth Laboratories , University of Edinburgh , Edinburgh , UK ; 2. b Edinburgh Medical School , University of Edinburgh , Edinburgh , UK ; 3. c Department of Pharmacology , Toxicology & Therapeutics, BHF Centre for Cardiovascular Science, The Queen's Medical Research Institute, University of Edinburgh , Edinburgh , UK.
Abstract
INTRODUCTION: MicroRNA 122 (miR-122) is a new circulating biomarker for liver injury, which increases earlier than conventional markers in patients with acetaminophen hepatotoxicity. However, as co-ingestion of ethanol is common with drug overdose, a confounding effect of acute ethanol consumption on serum miR-122 must be examined. METHODS: Blood was collected from healthy volunteers before and after recreational consumption of ethanol. Routine biochemistry and haematology measurements were performed, and serum miR-122 was measured by qPCR. The primary outcome was the difference in serum miR-122 with ethanol consumption. RESULTS: We recruited 18 participants (72% male). Their mean serum ethanol concentration was 113 mg/dl (95% confidence interval [CI] 91-135 mg/dl) after consuming ethanol. Serum miR-122 increased from a mean of 71.3 million (95% CI 29.3-113.2 million) to 139.1 million (95% CI 62.6-215.7 million) copies/ml (2.2-fold increase). There was no significant difference in serum alanine aminotransferase activity before and after ethanol consumption. CONCLUSION: miR-122 increased with moderate ethanol consumption, but the fold change was modest. As increases with acetaminophen toxicity are 100- to 10 000-fold, moderate ethanol intoxication is unlikely to confound the use of this biomarker of hepatotoxicity.
INTRODUCTION: MicroRNA 122 (miR-122) is a new circulating biomarker for liver injury, which increases earlier than conventional markers in patients with acetaminophenhepatotoxicity. However, as co-ingestion of ethanol is common with drug overdose, a confounding effect of acute ethanol consumption on serum miR-122 must be examined. METHODS: Blood was collected from healthy volunteers before and after recreational consumption of ethanol. Routine biochemistry and haematology measurements were performed, and serum miR-122 was measured by qPCR. The primary outcome was the difference in serum miR-122 with ethanol consumption. RESULTS: We recruited 18 participants (72% male). Their mean serum ethanol concentration was 113 mg/dl (95% confidence interval [CI] 91-135 mg/dl) after consuming ethanol. Serum miR-122 increased from a mean of 71.3 million (95% CI 29.3-113.2 million) to 139.1 million (95% CI 62.6-215.7 million) copies/ml (2.2-fold increase). There was no significant difference in serum alanine aminotransferase activity before and after ethanol consumption. CONCLUSION:miR-122 increased with moderate ethanol consumption, but the fold change was modest. As increases with acetaminophentoxicity are 100- to 10 000-fold, moderate ethanol intoxication is unlikely to confound the use of this biomarker of hepatotoxicity.
Authors: Emma Raitoharju; Ilkka Seppälä; Leo-Pekka Lyytikäinen; Jorma Viikari; Mika Ala-Korpela; Pasi Soininen; Antti J Kangas; Melanie Waldenberger; Norman Klopp; Thomas Illig; Jaana Leiviskä; Britt-Marie Loo; Niku Oksala; Mika Kähönen; Nina Hutri-Kähönen; Reijo Laaksonen; Olli Raitakari; Terho Lehtimäki Journal: Sci Rep Date: 2016-12-05 Impact factor: 4.379
Authors: James W Dear; Joanna I Clarke; Ben Francis; Lowri Allen; Jonathan Wraight; Jasmine Shen; Paul I Dargan; David Wood; Jamie Cooper; Simon H L Thomas; Andrea L Jorgensen; Munir Pirmohamed; B Kevin Park; Daniel J Antoine Journal: Lancet Gastroenterol Hepatol Date: 2017-11-14
Authors: W Oosthuyzen; P W L Ten Berg; B Francis; S Campbell; V Macklin; E Milne; A G Gow; C Fisher; R J Mellanby; J W Dear Journal: J Vet Intern Med Date: 2018-08-02 Impact factor: 3.333