Literature DB >> 2657098

Artificial cleavage site recognized by plum pox potyvirus protease in Escherichia coli.

J A García1, J L Riechmann, S Laín.   

Abstract

A synthetic plum pox virus (PPV) NIb-CP cleavage site was recognized by a PPV protease in an in vivo Escherichia coli expression system. The presence of the natural NIb-CP cleavage site did not affect processing at the artificial one. However, although both the proteases and the cleavage sites of PPV and tobacco etch virus show high sequence homology, a similar cassette from the tobacco etch virus NIb-CP junction was not efficiently recognized by the PPV protease.

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Year:  1989        PMID: 2657098      PMCID: PMC250700     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  7 in total

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Journal:  Biotechniques       Date:  1988-06       Impact factor: 1.993

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Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

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Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-encoded protease.

Authors:  G M Hellmann; J G Shaw; R E Rhoads
Journal:  Virology       Date:  1988-04       Impact factor: 3.616

6.  A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing.

Authors:  J C Carrington; W G Dougherty
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

7.  Biochemical and mutational analysis of a plant virus polyprotein cleavage site.

Authors:  W G Dougherty; J C Carrington; S M Cary; T D Parks
Journal:  EMBO J       Date:  1988-05       Impact factor: 11.598

  7 in total
  9 in total

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Journal:  Protein Cell       Date:  2012-10-24       Impact factor: 14.870

2.  Nucleotide sequence of the coat protein coding region of tulip breaking virus.

Authors:  K Ohira; S Namba; M Miyagawa; T Kusumi; T Tsuchizaki
Journal:  Virus Genes       Date:  1994-03       Impact factor: 2.332

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Authors:  F Regner; A da Câmara Machado; M L da Câmara Machado; H Steinkellner; D Mattanovich; V Hanzer; H Weiss; H Katinger
Journal:  Plant Cell Rep       Date:  1992-02       Impact factor: 4.570

4.  Nucleotide sequence of pea seed-borne mosaic potyvirus coat protein gene.

Authors:  E Johansen; O F Rasmussen; M Heide; B Borkhardt
Journal:  Virus Genes       Date:  1991-10       Impact factor: 2.332

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7.  Specific and efficient cleavage of fusion proteins by recombinant plum pox virus NIa protease.

Authors:  Nuoyan Zheng; José de Jesús Pérez; Zhonghui Zhang; Elvira Domínguez; Juan Antonio Garcia; Qi Xie
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8.  Inhibitory effects of human cystatin C on plum pox potyvirus proteases.

Authors:  J A García; M T Cervera; J L Riechmann; C López-Otín
Journal:  Plant Mol Biol       Date:  1993-07       Impact factor: 4.076

9.  Digitalizing heterologous gene expression in Gram-negative bacteria with a portable ON/OFF module.

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  9 in total

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