Literature DB >> 2656750

Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.

G J Delisle1, A Ley.   

Abstract

A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to 90%, and the specificity was 100%.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2656750      PMCID： PMC267418          DOI： 10.1128/jcm.27.4.778-779.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases.

Authors:  M Kilian; P Bülow
Journal:  Acta Pathol Microbiol Scand B       Date:  1976-10

2.  PRESENTATION, DIAGNOSIS, AND TREATMENT OF URINARY-TRACT INFECTIONS IN GENERAL PRACTICE.

Authors:  N C MOND; A PERCIVAL; J D WILLIAMS; W BRUMFITT
Journal:  Lancet       Date:  1965-03-06       Impact factor: 79.321

3.  Biochemical identification of hospital enterobacteria by replica agar plating.

Authors:  P Chadwick; G J Delisle; M Byer
Journal:  Can J Microbiol       Date:  1974-12       Impact factor: 2.419

4.  Indoxyl-beta-D-glucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples.

Authors:  A N Ley; R J Bowers; S Wolfe
Journal:  Can J Microbiol       Date:  1988-05       Impact factor: 2.419

5.  Fluorogenic assays for immediate confirmation of Escherichia coli.

Authors:  P C Feng; P A Hartman
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

6.  Comparison of beta-glucuronidase-based substrate systems for identification of Escherichia coli.

Authors:  S C Edberg; C M Kontnick
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

7.  Rapid and economical identification and antimicrobial susceptibility test methodology for urinary tract pathogens.

Authors:  S C Edberg; R W Trepeta
Journal:  J Clin Microbiol       Date:  1983-12       Impact factor: 5.948

8.  Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli.

Authors:  R W Trepeta; S C Edberg
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

9.  Rapid identification of Enterobacteriaceae. II. Use of a beta-glucuronidase detecting agar medium (PGUA agar) for the identification of E. coli in primary cultures of urine samples.

Authors:  M Kilian; P Bülow
Journal:  Acta Pathol Microbiol Scand B       Date:  1979-10

10.  Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis.

Authors:  M P Doyle; J L Schoeni
Journal:  Appl Environ Microbiol       Date:  1984-10       Impact factor: 4.792
View more
  19 in total

1.  Cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of CHROMagar orientation medium in conjunction with simple biochemical tests.

Authors:  K Ohkusu
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

Review 2.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors:  M Manafi; W Kneifel; S Bascomb
Journal:  Microbiol Rev       Date:  1991-09

3.  Evaluation of CPS ID2 medium for diagnosis of urinary infections.

Authors:  M L Núñez; J Díaz; I Lorente; J Pérez; J Ruiz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-12       Impact factor: 3.267

4.  Comparison of two test kits for rapid identification of Escherichia coli by a beta-glucuronidase assay.

Authors:  H K Geiss
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1990-02       Impact factor: 3.267

5.  Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens.

Authors:  K A Hengstler; R Hammann; A M Fahr
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

6.  Evaluation of the DipStreak, a new device with an original streaking mechanism for detection, counting, and presumptive identification of urinary tract pathogens.

Authors:  Claudio Scarparo; Paola Piccoli; Paolo Ricordi; Mariuccia Scagnelli
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

7.  The gusBC genes of Escherichia coli encode a glucuronide transport system.

Authors:  Wei-Jun Liang; Kate J Wilson; Hao Xie; Jan Knol; Shun'ichi Suzuki; Nicholas G Rutherford; Peter J F Henderson; Richard A Jefferson
Journal:  J Bacteriol       Date:  2005-04       Impact factor: 3.490

8.  Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.

Authors:  H Kodaka; M Ishikawa; M Iwata; F Kashitani; S Mizuochi; K Yamaguchi
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

9.  Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli.

Authors:  J R Haines; T C Covert; C C Rankin
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

10.  Distribution of uidA gene sequences in Escherichia coli isolates in water sources and comparison with the expression of beta-glucuronidase activity in 4-methylumbelliferyl-beta-D-glucuronide media.

Authors:  M T Martins; I G Rivera; D L Clark; M H Stewart; R L Wolfe; B H Olson
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.