Lorena Gallego1, Marcos Pérez-Basterrechea2, Luis García-Consuegra3, María Álvarez-Viejo2, Joaquim Megías3, Alba Novoa3, Serafín Costilla4,5, Álvaro Meana2,6, Luis Junquera3,7. 1. Department of Oral and Maxillofacial Surgery, Cabueñes Hospital, Gijón, Spain. 2. Transplants, Cell Therapy and Regenerative Medicine Unit, Central University Hospital, Oviedo, Spain. 3. Department of Oral and Maxillofacial Surgery, Central University Hospital, Oviedo, Spain. 4. Department of Medicine, University of Oviedo, Oviedo, Spain. 5. Department of Radiology, Central University Hospital, Oviedo, Spain. 6. Ophthalmologic Research Foundation, U714, CIBER Rare Diseases (CIBERER), Oviedo, Spain. 7. Department of Surgery, University of Oviedo, Oviedo, Spain.
Abstract
AIM: The aim of this study was to evaluate effective bone regeneration using an autologous serum scaffold (alone or seeded with autologous bone marrow-mesenchymal stem cells, BM-MSCs), when implanted in a 30 mm length segmental mandibular defect in sheep. MATERIALS AND METHODS: The bone defect was filled either with serum scaffold alone (control group; n = 5) or combined with BM-MSCs (experimental group; n = 10). Bone regeneration was determined at 12 (T12; 2 control sheep and 4 experimental sheep) and 32 weeks (T32; 3 control and 6 experimental sheep), as measured by computed and microcomputed tomography and histological examination. RESULTS: Two sheep of the Experimental group died after surgery. While complete bone union in the control group was only observed at T32, it was observed both at T12 (1/4 sheep) and T32 (3/4 sheep) in the experimental group. When properties/characteristics of new bone where compared, a better bone quality, similar to native bone, was observed in the scaffold combined with BM-MSCs. CONCLUSIONS: Based on these results, we conclude that the serum scaffold can promote efficient repair of large bone defects, but the combination with BM-MSCs accelerates this process, increasing significantly the amount and quality of bone formed.
AIM: The aim of this study was to evaluate effective bone regeneration using an autologous serum scaffold (alone or seeded with autologous bone marrow-mesenchymal stem cells, BM-MSCs), when implanted in a 30 mm length segmental mandibular defect in sheep. MATERIALS AND METHODS: The bone defect was filled either with serum scaffold alone (control group; n = 5) or combined with BM-MSCs (experimental group; n = 10). Bone regeneration was determined at 12 (T12; 2 control sheep and 4 experimental sheep) and 32 weeks (T32; 3 control and 6 experimental sheep), as measured by computed and microcomputed tomography and histological examination. RESULTS: Two sheep of the Experimental group died after surgery. While complete bone union in the control group was only observed at T32, it was observed both at T12 (1/4 sheep) and T32 (3/4 sheep) in the experimental group. When properties/characteristics of new bone where compared, a better bone quality, similar to native bone, was observed in the scaffold combined with BM-MSCs. CONCLUSIONS: Based on these results, we conclude that the serum scaffold can promote efficient repair of large bone defects, but the combination with BM-MSCs accelerates this process, increasing significantly the amount and quality of bone formed.
Authors: C Black; J M Kanczler; M C de Andrés; L J White; F M Savi; O Bas; S Saifzadeh; J Henkel; A Zannettino; S Gronthos; M A Woodruff; D W Hutmacher; R O C Oreffo Journal: Biomaterials Date: 2020-04-01 Impact factor: 12.479
Authors: Simon Spalthoff; Rüdiger Zimmerer; Jan Dittmann; Horst Kokemüller; Marco Tiede; Laura Flohr; Philippe Korn; Nils-Claudius Gellrich; Philipp Jehn Journal: Regen Biomater Date: 2017-11-27
Authors: Maria Rosa Iaquinta; Elisa Mazzoni; Ilaria Bononi; John Charles Rotondo; Chiara Mazziotta; Monica Montesi; Simone Sprio; Anna Tampieri; Mauro Tognon; Fernanda Martini Journal: Front Cell Dev Biol Date: 2019-11-12