Draft Genome Sequence of Bacillus tequilensis Strain FJAT-14262a.

Bacillus tequilensis FJAT-14262a is a Gram-positive rod-shaped bacterium. Here, we report the 4,038,551-bp genome sequence of B. tequilensis FJAT-14262a, which will provide useful information for genomic taxonomy and phylogenomics of Bacillus.

GENOME ANNOUNCEMENT

The genus Bacillus is the most diverse and prominent genus of aerobic endospore-forming bacteria (1), consisting of ~299 species and 7 subspecies until 2015 (List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.net/bacillus.html). Some Bacillus components have important roles in industrial processes, such as the transglutaminase of Bacillus subtilis and biofilm formation of butyric acid (2, 3). Bacillus strains are widely distributed in the environment. Strain FJAT-14262a was isolated from rhizosphere soil of Anoectochilus roxburghii in China and identified as Bacillus tequilensis, which is a phylogenetically close neighbor of B. subtilis according to their 16S rRNA gene sequence similarity. The best growth of B. tequilensis FJAT-14262a was achieved at 35°C and pH 8. Here, we present the high-quality draft genome sequence of B. tequilensis FJAT-14262a.

The genome sequencing of B. tequilensis FJAT-14262a was performed via the Illumina HiSeq 2500 system. Two DNA libraries with insert sizes of 500 and 5,000 bp were constructed and sequenced. The genome coverage was approximately 150-fold coverage. The reads were assembled via the SOAPdenovo software version 2.10 (4), using a key parameter K setting at 77. Through the data assembly, 7 scaffolds with a total length of 3,853,296 bp were obtained, and the contig N50 was 185,225 bp. The average length of the scaffolds was 550,470 bp, and the longest and shortest scaffolds were 1,047,314 bp and 711 bp, respectively. A total 79.77% clean reads were aligned back to the genome, which covered 99.57% of the sequence.

Annotation of the genome was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) (http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html) utilizing the GeneMark, Glimmer, and tRNAscan-SE tools (5). A total of 4,029 genes were predicted, including 3,919 coding sequences (CDSs), 41 pseudogenes, 66 tRNAs, 6 rRNAs, and 1 noncoding RNA (ncRNA) gene. There were 3,090 genes assigned to the COG database. The average DNA G+C content was 43.71%.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession no. LGRW00000000. The version described in this paper is version LGRW00000000.1.