OBJECTIVE: The longer the Pericarpium Citri Reticulatae is preserved, the better medicinal values will be. The present work aims to isolate beneficial microbes isolated from the 7-year orange peel that might be used to produce high quality Pericarpium Citri Reticulatae. METHODS: The microbes isolated from the 7-year orange peel using 4 mediums were grouped by SDS-PAGE patterns of the whole-cell protein electrophoresis and IS-PCR DNA fingerprinting. The representative strains were further studied by physiological and biochemical tests, and phylogeny analysis. RESULTS: Total 23 bacteria were obtained from the 7-year orange peel. These strains were classified into 4 groups: the strains of group I belonged to Bacillus; the strains of group II, group III and group IV were closed to Paenibacillus. CONCLUSION: Among the representative strains of group I, II, III and IV, only the representative strain cp20 of group II has the ability of strong utilizing citrate obviously. The strain cp20 of group II were further applied to making Pericarpium Citri Reticulatae.
OBJECTIVE: The longer the Pericarpium Citri Reticulatae is preserved, the better medicinal values will be. The present work aims to isolate beneficial microbes isolated from the 7-year orange peel that might be used to produce high quality Pericarpium Citri Reticulatae. METHODS: The microbes isolated from the 7-year orange peel using 4 mediums were grouped by SDS-PAGE patterns of the whole-cell protein electrophoresis and IS-PCR DNA fingerprinting. The representative strains were further studied by physiological and biochemical tests, and phylogeny analysis. RESULTS: Total 23 bacteria were obtained from the 7-year orange peel. These strains were classified into 4 groups: the strains of group I belonged to Bacillus; the strains of group II, group III and group IV were closed to Paenibacillus. CONCLUSION: Among the representative strains of group I, II, III and IV, only the representative strain cp20 of group II has the ability of strong utilizing citrate obviously. The strain cp20 of group II were further applied to making Pericarpium Citri Reticulatae.