Literature DB >> 2656287

Novel and sensitive noncompetitive enzyme immunoassay for kassinin in rat plasma.

K Tanaka1, S Hashida, T Kohno, Z Mohri, Y Murakami, E Ishikawa.   

Abstract

A novel and sensitive noncompetitive enzyme immunoassay for kassinin is described. Kassinin was biotinylated using sulfosuccinimidyl-6-(biotinamido)hexanoate. The biotinylated kassinin was trapped on anti-kassinin IgG-coated polystyrene balls and, after washing to eliminate other biotinylated substances, was eluted with HCl. The biotinylated kassinin eluted was reacted with anti-kassinin Fab'-peroxidase conjugate and trapped onto streptavidin-coated polystyrene balls. Peroxidase activity bound to the polystyrene balls was assayed by fluorimetry. The detection limit of kassinin was 0.13 pg (0.1 fmol)/tube or 0.065 microgram/l of rat plasma, which was 750-fold or 15-fold lower than that for competitive radioimmunoassay.

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Year:  1989        PMID： 2656287     DOI： 10.1007/bf01952033

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  8 in total

1.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors:  F C GREENWOOD; W M HUNTER; J S GLOVER
Journal:  Biochem J       Date:  1963-10       Impact factor: 3.857

2.  Amino acid composition and sequence of kassinin, a tachykinin dodecapeptide from the skin of the African frog Kassina senegalensis.

Authors:  A Anastasi; P Montecucchi; V Erspamer; J Visser
Journal:  Experientia       Date:  1977-07-15

3.  A micro-scale affinity-purification of Fab'-horseradish peroxidase conjugates and its use for sandwich enzyme immunoassay of insulin in human serum.

Authors:  K Ruan; S Hashida; S Yoshitake; E Ishikawa; O Wakisaka; Y Yamamoto; T Ichioka; K Nakajima
Journal:  Clin Chim Acta       Date:  1985-04-15       Impact factor: 3.786

Review 4.  Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining.

Authors:  E Ishikawa; M Imagawa; S Hashida; S Yoshitake; Y Hamaguchi; T Ueno
Journal:  J Immunoassay       Date:  1983

5.  Studies on peptides. LXXVI. Synthesis of kassinin, a new frog skin peptide.

Authors:  H Yajima; T Sasaki; H Ogawa; N Juji; T Segawa; Y Nakata
Journal:  Chem Pharm Bull (Tokyo)       Date:  1978-04       Impact factor: 1.645

6.  Growth hormone, prolactin and FSH release by the tachykinin dodecapeptide kassinin in the rat.

Authors:  H G Güllner; H Yajima; D Herbert; W W Owen
Journal:  Arch Int Pharmacodyn Ther       Date:  1982-03

7.  New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates.

Authors:  F T Liu; M Zinnecker; T Hamaoka; D H Katz
Journal:  Biochemistry       Date:  1979-02-20       Impact factor: 3.162

8.  More useful maleimide compounds for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge.

Authors:  S Hashida; M Imagawa; S Inoue; K H Ruan; E Ishikawa
Journal:  J Appl Biochem       Date:  1984 Feb-Apr
  8 in total

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