Zhihong Qi1, Yu Chen1, Lin Zhang1, Xinming Ma2, Fei Wang1, Qian Cheng1, Juan Du1, Yingying Hao1, Shuling Chi1, Wei Cui3. 1. Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China. 2. Institute of Basic Medicine Sciences, Chinese Academy of Medical Sciences School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China. 3. Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China. Electronic address: wendycuiwei@sina.cn.
Abstract
BACKGROUND: Reports on biological variation of plasma biochemical indicators are limited. We evaluated biological variations of 13 plasma biochemical indicators. METHODS: Plasma samples were collected from 40 healthy individuals over 5days. Intra-individual coefficient of variation (CVI), inter-individual coefficient of variation (CVG), index of individuality (II), reference change value (RCV), and analytical goal parameters were calculated. RESULTS: Albumin (Alb) showed the lowest CVI (2.50%) and the lowest CVG (5.08%), while C-reactive protein (hsCRP) presented the highest CVI (26.87%) and CVG (61.73%). II values were all less than 1.0. Alb presented the lowest 95% RCV (7.67), while hsCRP showed the highest 95% RCV (74.61). Alb, urea, creatinine (Cr), creatine kinase (CK), and creatine kinase isoenzyme MB (CKMBmass) CVI differed with gender (P<0.05). The CVG of the 13 indicators presented a significant gender difference (P<0.0001). Alb showed the lowest desirable imprecision CV (1.3%), the lowest desirable bias (1.4%), and the lowest desirable total error (3.5%), while hsCRP presented the highest desirable imprecision (13.4%), the highest desirable bias (16.8%), and the highest desirable total error (39.0%). CONCLUSION: Our findings add to the database of biological variations of plasma indicators.
BACKGROUND: Reports on biological variation of plasma biochemical indicators are limited. We evaluated biological variations of 13 plasma biochemical indicators. METHODS: Plasma samples were collected from 40 healthy individuals over 5days. Intra-individual coefficient of variation (CVI), inter-individual coefficient of variation (CVG), index of individuality (II), reference change value (RCV), and analytical goal parameters were calculated. RESULTS:Albumin (Alb) showed the lowest CVI (2.50%) and the lowest CVG (5.08%), while C-reactive protein (hsCRP) presented the highest CVI (26.87%) and CVG (61.73%). II values were all less than 1.0. Alb presented the lowest 95% RCV (7.67), while hsCRP showed the highest 95% RCV (74.61). Alb, urea, creatinine (Cr), creatine kinase (CK), and creatine kinase isoenzyme MB (CKMBmass) CVI differed with gender (P<0.05). The CVG of the 13 indicators presented a significant gender difference (P<0.0001). Alb showed the lowest desirable imprecision CV (1.3%), the lowest desirable bias (1.4%), and the lowest desirable total error (3.5%), while hsCRP presented the highest desirable imprecision (13.4%), the highest desirable bias (16.8%), and the highest desirable total error (39.0%). CONCLUSION: Our findings add to the database of biological variations of plasma indicators.