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Literature DB >> 26559199

Retina-on-a-chip: a microfluidic platform for point access signaling studies.

Kirsten H Dodson¹, Franklin D Echevarria², Deyu Li³, Rebecca M Sappington⁴, Jon F Edd^5,6.

Abstract

We report on a microfluidic platform for culture of whole organs or tissue slices with the capability of point access reagent delivery to probe the transport of signaling events. Whole mice retina were maintained for multiple days with negative pressure applied to tightly but gently bind the bottom of the retina to a thin poly-(dimethylsiloxane) membrane, through which twelve 100 μm diameter through-holes served as fluidic access points. Staining with toluidine blue, transport of locally applied cholera toxin beta, and transient response to lipopolysaccharide in the retina demonstrated the capability of the microfluidic platform. The point access fluidic delivery capability could enable new assays in the study of various kinds of excised tissues, including retina.

Entities: Chemical Disease Gene Species

Keywords: Microenvironment; Microfluidic tissue culture; Retina

Mesh：

Year: 2015 PMID： 26559199 PMCID： PMC4707151 DOI： 10.1007/s10544-015-0019-x

Source DB: PubMed Journal: Biomed Microdevices ISSN： 1387-2176 Impact factor: 2.838

41 in total

1. A microfluidic culture platform for CNS axonal injury, regeneration and transport.

Authors: Anne M Taylor; Mathew Blurton-Jones; Seog Woo Rhee; David H Cribbs; Carl W Cotman; Noo Li Jeon
Journal: Nat Methods Date: 2005-08 Impact factor: 28.547

2. Interleukin-6 protects retinal ganglion cells from pressure-induced death.

Authors: Rebecca M Sappington; Matilda Chan; David J Calkins
Journal: Invest Ophthalmol Vis Sci Date: 2006-07 Impact factor: 4.799

3. Bacterial lipopolysaccharide selectively up-regulates the function of the chemotactic peptide receptor formyl peptide receptor 2 in murine microglial cells.

Authors: You-Hong Cui; Yingying Le; Wanghua Gong; Paul Proost; Jo Van Damme; William J Murphy; Ji Ming Wang
Journal: J Immunol Date: 2002-01-01 Impact factor: 5.422

4. Spatial regulation of interleukin-6 signaling in response to neurodegenerative stressors in the retina.

Authors: Stephanie M Sims; Lauren Holmgren; Heather M Cathcart; Rebecca M Sappington
Journal: Am J Neurodegener Dis Date: 2012

5. A simple method for fabricating multi-layer PDMS structures for 3D microfluidic chips.

Authors: Mengying Zhang; Jinbo Wu; Limu Wang; Kang Xiao; Weijia Wen
Journal: Lab Chip Date: 2010-02-09 Impact factor: 6.799

6. Lateral diffusion of lipopolysaccharide in the outer membrane of Salmonella typhimurium.

Authors: M Schindler; M J Osborn; D E Koppel
Journal: Nature Date: 1980-05-22 Impact factor: 49.962

7. Simplified equation to extract diffusion coefficients from confocal FRAP data.

Authors: Minchul Kang; Charles A Day; Anne K Kenworthy; Emmanuele DiBenedetto
Journal: Traffic Date: 2012-10-10 Impact factor: 6.215

8. Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures.

Authors: Ryan J Horvath; Nancy Nutile-McMenemy; Matthew S Alkaitis; Joyce A Deleo
Journal: J Neurochem Date: 2008-09-18 Impact factor: 5.372

9. Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane.

Authors: K Triantafilou; M Triantafilou; S Ladha; A Mackie; R L Dedrick; N Fernandez; R Cherry
Journal: J Cell Sci Date: 2001-07 Impact factor: 5.285

10. The optic nerve head is the site of axonal transport disruption, axonal cytoskeleton damage and putative axonal regeneration failure in a rat model of glaucoma.

Authors: Glyn Chidlow; Andreas Ebneter; John P M Wood; Robert J Casson
Journal: Acta Neuropathol Date: 2011-02-11 Impact factor: 17.088

16 in total

Retina-on-a-chip: a microfluidic platform for point access signaling studies.

1. A microfluidic culture platform for CNS axonal injury, regeneration and transport.

2. Interleukin-6 protects retinal ganglion cells from pressure-induced death.

3. Bacterial lipopolysaccharide selectively up-regulates the function of the chemotactic peptide receptor formyl peptide receptor 2 in murine microglial cells.

4. Spatial regulation of interleukin-6 signaling in response to neurodegenerative stressors in the retina.

5. A simple method for fabricating multi-layer PDMS structures for 3D microfluidic chips.

6. Lateral diffusion of lipopolysaccharide in the outer membrane of Salmonella typhimurium.

7. Simplified equation to extract diffusion coefficients from confocal FRAP data.

8. Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures.

9. Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane.

10. The optic nerve head is the site of axonal transport disruption, axonal cytoskeleton damage and putative axonal regeneration failure in a rat model of glaucoma.

Review 1. Ex Vivo Tumor-on-a-Chip Platforms to Study Intercellular Interactions within the Tumor Microenvironment.

2. From Engineered Tissues and Microfludics to Human Eyes-On-A-Chip.

3. The relationship between the Young's modulus and dry etching rate of polydimethylsiloxane (PDMS).

Review 4. Bridging the gap: microfluidic devices for short and long distance cell-cell communication.

Review 5. Role of In Vitro Models for Development of Ophthalmic Delivery Systems.

Review 6. Microfabricated Physiological Models for In Vitro Drug Screening Applications.

Review 7. Conceptual Design of Micro-Bioreactors and Organ-on-Chips for Studies of Cell Cultures.

8. Maintenance of head and neck tumor on-chip: gateway to personalized treatment?

9. A Micro-Optic Stalk (μOS) System to Model the Collective Migration of Retinal Neuroblasts.

Review 10. Advanced Fabrication Techniques of Microengineered Physiological Systems.