AIM: To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression. METHODS: Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed. RESULTS: The bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05). CONCLUSION: The expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.
AIM: To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression. METHODS: Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed. RESULTS: The bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05). CONCLUSION: The expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.
Authors: Ani V Das; Kavita B Mallya; Xing Zhao; Faraz Ahmad; Sumitra Bhattacharya; Wallace B Thoreson; Ganapati V Hegde; Iqbal Ahmad Journal: Dev Biol Date: 2006-07-29 Impact factor: 3.582
Authors: Andreas Bringmann; Thomas Pannicke; Jens Grosche; Mike Francke; Peter Wiedemann; Serguei N Skatchkov; Neville N Osborne; Andreas Reichenbach Journal: Prog Retin Eye Res Date: 2006-07-12 Impact factor: 21.198
Authors: Virginie J M Verhoeven; Pirro G Hysi; Robert Wojciechowski; Qiao Fan; Jeremy A Guggenheim; René Höhn; Stuart MacGregor; Alex W Hewitt; Abhishek Nag; Ching-Yu Cheng; Ekaterina Yonova-Doing; Xin Zhou; M Kamran Ikram; Gabriëlle H S Buitendijk; George McMahon; John P Kemp; Beate St Pourcain; Claire L Simpson; Kari-Matti Mäkelä; Terho Lehtimäki; Mika Kähönen; Andrew D Paterson; S Mohsen Hosseini; Hoi Suen Wong; Liang Xu; Jost B Jonas; Olavi Pärssinen; Juho Wedenoja; Shea Ping Yip; Daniel W H Ho; Chi Pui Pang; Li Jia Chen; Kathryn P Burdon; Jamie E Craig; Barbara E K Klein; Ronald Klein; Toomas Haller; Andres Metspalu; Chiea-Chuen Khor; E-Shyong Tai; Tin Aung; Eranga Vithana; Wan-Ting Tay; Veluchamy A Barathi; Peng Chen; Ruoying Li; Jiemin Liao; Yingfeng Zheng; Rick T Ong; Angela Döring; David M Evans; Nicholas J Timpson; Annemieke J M H Verkerk; Thomas Meitinger; Olli Raitakari; Felicia Hawthorne; Tim D Spector; Lennart C Karssen; Mario Pirastu; Federico Murgia; Wei Ang; Aniket Mishra; Grant W Montgomery; Craig E Pennell; Phillippa M Cumberland; Ioana Cotlarciuc; Paul Mitchell; Jie Jin Wang; Maria Schache; Sarayut Janmahasatian; Sarayut Janmahasathian; Robert P Igo; Jonathan H Lass; Emily Chew; Sudha K Iyengar; Theo G M F Gorgels; Igor Rudan; Caroline Hayward; Alan F Wright; Ozren Polasek; Zoran Vatavuk; James F Wilson; Brian Fleck; Tanja Zeller; Alireza Mirshahi; Christian Müller; André G Uitterlinden; Fernando Rivadeneira; Johannes R Vingerling; Albert Hofman; Ben A Oostra; Najaf Amin; Arthur A B Bergen; Yik-Ying Teo; Jugnoo S Rahi; Veronique Vitart; Cathy Williams; Paul N Baird; Tien-Yin Wong; Konrad Oexle; Norbert Pfeiffer; David A Mackey; Terri L Young; Cornelia M van Duijn; Seang-Mei Saw; Joan E Bailey-Wilson; Dwight Stambolian; Caroline C Klaver; Christopher J Hammond Journal: Nat Genet Date: 2013-02-10 Impact factor: 38.330