Mariam Omer Bin Hamza1, Khalid Gufran2, Kusai Baroudi3. 1. Lecturer, Department of Restorative Dental Sciences, Al-Farabi Colleges , Riyadh, Saudi Arabia . 2. Lecturer, Department of Preventive Dental Sciences, College of Dentistry, Salman Bin Abdul Aziz University , Al-Kharj, Saudi Arabia . 3. Assistant Professor, Department of Preventive Dental Sciences, Al-Farabi Colleges , Riyadh, Saudi Arabia .
Abstract
INTRODUCTION: Obturating materials exposed to the dental operating environment has been shown to be contaminated, making rapid chair side disinfection mandatory to ensure the sterility of the root canals. OBJECTIVE: The purpose of this study was to investigate the effectiveness of CFC (combination of Calcium hydroxide, Flagyl and Ciprofloxacin) for the rapid disinfection of Gutta-percha and Resilon cones. MATERIALS AND METHODS: Seventy new Gutta-percha and Resilon cones were randomly selected, contaminated with Enterococcus faecalis and divided into 4 group according to the irrigant used for disinfection {Group I: 5.25% Sodium Hypochlorite (NaOCl), Group II: MTAD, Group III: 2% Chlorhexidine (CHX), Group IV: CFC (a combination of Calcium hydroxide, Flagyl and Ciprofloxacin)}. All the samples were placed in a centrifuge tube with BHI broth after being washed with sterile water. The samples were then incubated for 7 days at 37(o)C. Samples were randomly plated on Mac Conkey agar plate and the colony count was recorded and the observations were drawn. RESULTS: A 5.25% NaOCl required 1 minute exposure for effective disinfection of all the samples. MTAD could eliminate E.faecalis from gutta-percha samples in 30 seconds whereas it required 1 minute of exposure for Resilon cones. Both 2% CHX and CFC could not disinfect the samples with 1 minute exposure and a minimum of 5 minute exposure was required. CONCLUSION: A 5.25% NaOCl and Biopure MTAD required less chair side time to disinfect all the samples effectively when compared with 2% CHX and CFC.
INTRODUCTION: Obturating materials exposed to the dental operating environment has been shown to be contaminated, making rapid chair side disinfection mandatory to ensure the sterility of the root canals. OBJECTIVE: The purpose of this study was to investigate the effectiveness of CFC (combination of Calcium hydroxide, Flagyl and Ciprofloxacin) for the rapid disinfection of Gutta-percha and Resilon cones. MATERIALS AND METHODS: Seventy new Gutta-percha and Resilon cones were randomly selected, contaminated with Enterococcus faecalis and divided into 4 group according to the irrigant used for disinfection {Group I: 5.25% Sodium Hypochlorite (NaOCl), Group II: MTAD, Group III: 2% Chlorhexidine (CHX), Group IV: CFC (a combination of Calcium hydroxide, Flagyl and Ciprofloxacin)}. All the samples were placed in a centrifuge tube with BHI broth after being washed with sterile water. The samples were then incubated for 7 days at 37(o)C. Samples were randomly plated on Mac Conkey agar plate and the colony count was recorded and the observations were drawn. RESULTS: A 5.25% NaOCl required 1 minute exposure for effective disinfection of all the samples. MTAD could eliminate E.faecalis from gutta-percha samples in 30 seconds whereas it required 1 minute of exposure for Resilon cones. Both 2% CHX and CFC could not disinfect the samples with 1 minute exposure and a minimum of 5 minute exposure was required. CONCLUSION: A 5.25% NaOCl and Biopure MTAD required less chair side time to disinfect all the samples effectively when compared with 2% CHX and CFC.
Entities:
Keywords:
Antimicrobial agent; E.faecalis; Prime root canal filling material
Authors: P G da Motta; C B de Figueiredo; S M Maltos; J R Nicoli; A P Ribeiro Sobrinho; K L Maltos; H P Carvalhais Journal: Int Endod J Date: 2001-09 Impact factor: 5.264
Authors: Daniel P Oliveira; Joao V B Barbizam; Martin Trope; Fabricio B Teixeira Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Date: 2007-03-26