Hemalatha Anthanahalli Lingappa1, Abhishek Mandya Govindashetty2, Anoosha Krishnamurthy3, Ashok Kagathur Puttaveerachary3, Sanjay Manchaiah4, Indira Channagangappa Shimoga5, Sushma Hulikere Mallaradhya3, Sarvesh Ballekoppa Mukunda Gowda6. 1. Professor and HOD, Department of Pathology, Adichunchanagiri Institute of Medical Sciences , B. G. Nagar, Mandya District, Karnataka, India . 2. Associate Professor, Department of Pathology, Adichunchanagiri Institute of Medical Sciences , B. G. Nagar, Mandya District, Karnataka, India . 3. Post Graduate, Department of Pathology, Adichunchanagiri Institute of Medical Sciences , B. G. Nagar, Mandya District, Karnataka, India . 4. Assistant Professor, Department of Pathology, Adichunchanagiri Institute of Medical Sciences , B. G. Nagar, Mandya District, Karnataka, India . 5. Tutor, Department of Pathology, Adichunchanagiri Institute of Medical Sciences , B. G. Nagar, Mandya District, Karnataka, India . 6. Assistant Professor, Department of Pathology, Affiliated to Rajiv Gandhi University of Health Sciences , Karnataka, India .
Abstract
BACKGROUND: Recent alarming trends of a substantial rise in the number of cases of infertility with as many as 30-40% being attributed to male-factor associated causes have created a need for further studies and advancements in semen analysis. Despite the focus on semen analysis over the years, assessment of sperm morphology has not been given due importance although it is a simple, standard and baseline diagnostic modality. It can be used to predict the need and outcome of Artificial Reproductive Techniques such as Invitro Fertilization, Gamete Intra Fallopian Tube Transfer and Intra Cytoplasmic Sperm Injection. AIM: To find the ideal, simple and cost-effective basic stain for assessment of sperm morphology in a rural tertiary care set- up where advanced equipment for assessment of sperm morphometry are inaccessible. MATERIALS AND METHODS: An updated way of determining sperm shape is called the Kruger's strict morphology method. Keeping this as the standard criterion, we studied semen samples of 62 healthy male subjects using four basic staining techniques and the consensus of four independent observers was tabulated. RESULTS: We found that Haematoxylin and Eosin stain was the best stain for assessment of sperm head morphology. Rapid Papanicolau stain was the most ideal, simple and cost-effective stain for overall assessment of sperm morphology. CONCLUSION: Sperm morphology assessment remains the baseline necessity for the diagnosis and management of male factor associated infertility when advanced techniques are unavailable, inaccessible or unaffordable.
BACKGROUND: Recent alarming trends of a substantial rise in the number of cases of infertility with as many as 30-40% being attributed to male-factor associated causes have created a need for further studies and advancements in semen analysis. Despite the focus on semen analysis over the years, assessment of sperm morphology has not been given due importance although it is a simple, standard and baseline diagnostic modality. It can be used to predict the need and outcome of Artificial Reproductive Techniques such as Invitro Fertilization, Gamete Intra Fallopian Tube Transfer and Intra Cytoplasmic Sperm Injection. AIM: To find the ideal, simple and cost-effective basic stain for assessment of sperm morphology in a rural tertiary care set- up where advanced equipment for assessment of sperm morphometry are inaccessible. MATERIALS AND METHODS: An updated way of determining sperm shape is called the Kruger's strict morphology method. Keeping this as the standard criterion, we studied semen samples of 62 healthy male subjects using four basic staining techniques and the consensus of four independent observers was tabulated. RESULTS: We found that Haematoxylin and Eosin stain was the best stain for assessment of sperm head morphology. Rapid Papanicolau stain was the most ideal, simple and cost-effective stain for overall assessment of sperm morphology. CONCLUSION: Sperm morphology assessment remains the baseline necessity for the diagnosis and management of male factor associated infertility when advanced techniques are unavailable, inaccessible or unaffordable.
Authors: D S Guzick; J W Overstreet; P Factor-Litvak; C K Brazil; S T Nakajima; C Coutifaris; S A Carson; P Cisneros; M P Steinkampf; J A Hill; D Xu; D L Vogel Journal: N Engl J Med Date: 2001-11-08 Impact factor: 91.245
Authors: Trevor G Cooper; Elizabeth Noonan; Sigrid von Eckardstein; Jacques Auger; H W Gordon Baker; Hermann M Behre; Trine B Haugen; Thinus Kruger; Christina Wang; Michael T Mbizvo; Kirsten M Vogelsong Journal: Hum Reprod Update Date: 2009-11-24 Impact factor: 15.610