Mauricio Baeza1,2, Mauricio Garrido1,2, Patricia Hernández-Ríos2, Andrea Dezerega1,2, Jocelyn García-Sesnich1, Franz Strauss2, Juan Pablo Aitken3, Emmanuel Lesaffre4, Sophie Vanbelle5, Jorge Gamonal1,2, Romina Brignardello-Petersen3, Taina Tervahartiala6, Timo Sorsa6,7, Marcela Hernández1,3. 1. Laboratory of Periodontal Biology, Department of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago, Chile. 2. Department of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago, Chile. 3. Department of Pathology and Oral Medicine, Faculty of Dentistry, University of Chile, Santiago, Chile. 4. Leuven Biostatistics and Statistical Bioinformatics Centre, KU Leuven, Leuven, Belgium. 5. Department of Methodology and Statistics, CAPHRI, Maastricht University, Maastricht, The Netherlands. 6. Departments of Oral and Maxillofacial Diseases, Helsinki University and Helsinki University Central Hospital, Helsinki, Finland. 7. Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
Abstract
AIM: The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis ( AAP). MATERIALS AND METHODS: Thirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAP patients and 31 GCF samples from 13 healthy volunteers were obtained (N = 106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNFα, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. RESULTS: The MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p < 0.05). ProMMP-2, MPO, IL-1, IL-6, PTN, TRAP-5 and OPG were significantly higher in CP when compared with AAP and healthy patients (p < 0.05). The highest diagnostic accuracies were observed for ProMMP-2, ProMMP-9, MMP-8 and TRAP-5 (AUC > 0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC > 0.90) in AAP. CONCLUSION: Gingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
AIM: The aim of this study was to assess the levels and diagnostic accuracy of a set of potential biomarkers of periodontal tissue metabolism in gingival crevicular fluid (GCF) from patients with chronic periodontitis (CP) and asymptomatic apical periodontitis ( AAP). MATERIALS AND METHODS: Thirty one GCF samples from 11 CP patients, 44 GCF samples from 38 AAPpatients and 31 GCF samples from 13 healthy volunteers were obtained (N = 106). Matrix metalloproteinases (MMPs) -2 and -9 were determined by zymography; levels of MMP-8 by ELISA and IFMA and MPO by ELISA. IL-1, IL-6, TNFα, DKK-1, Osteonectin, Periostin, TRAP-5 and OPG were determined by a multiplex quantitative panel. Statistical analysis was performed using linear mixed-effects models. RESULTS: The MMP-9 and MMP-8 were higher in CP, followed by AAP, versus healthy individuals (p < 0.05). ProMMP-2, MPO, IL-1, IL-6, PTN, TRAP-5 and OPG were significantly higher in CP when compared with AAP and healthy patients (p < 0.05). The highest diagnostic accuracies were observed for ProMMP-2, ProMMP-9, MMP-8 and TRAP-5 (AUC > 0.97) in CP, and for the active form of MMP-9 and MMP-8 (AUC > 0.90) in AAP. CONCLUSION: Gingival crevicular fluid composition is modified by CP and AAP. MMP-9 and MMP-8 show diagnostic potential for CP and AAP, whereas MMP-2 and TRAP-5 are useful only for CP.
Authors: Norma Samanta Romero-Castro; Mirna Vázquez-Villamar; José Francisco Muñoz-Valle; Salvador Reyes-Fernández; Victor Othón Serna-Radilla; Samuel García-Arellano; Natividad Castro-Alarcón Journal: Odontology Date: 2019-06-18 Impact factor: 2.634
Authors: M Salinas-Muñoz; M Garrido-Flores; M Baeza; P Huamán-Chipana; J García-Sesnich; R Bologna; R Vernal; M Hernández Journal: Clin Oral Investig Date: 2017-03-06 Impact factor: 3.573