Lacinutrix gracilariae sp. nov., isolated from the surface of a marine red alga Gracilaria sp.

A Gram-stain-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain Lxc1T, was isolated from the surface of a marine red alga, Gracilaria sp., which was collected from the coastal regions in Jinjiang, Fujian Province, China. Colonies of the strain were orange-yellow, circular and smooth. The 16S rRNA gene of strain Lxc1T had maximum sequence similarity with Lacinutrix himadriensis E4-9aT (97.1 %), followed by Lacinutrix jangbogonensis PAMC 27137T, Lacinutrix copepodicola DJ3T, Lacinutrix algicola AKS293T and Lacinutrix mariniflava AKS 432T (similarities < 96.4 %). Phylogenetic analysis showed strain Lxc1T formed a tight cluster with L. himadriensis E4-9aT and L. copepodicola DJ3T, but represented a novel lineage belonging to the genus Lacinutrix. The predominant fatty acids were iso-C15 : 1 G (18.3 %), iso-C15 : 0 (16.7 %), iso-C17 : 0 3-OH (10.6 %) and iso-C15 : 0 3-OH (8.6 %). Menaquinone-6 (MK-6) was the only respiratory quinone present. The DNA G+C content of strain Lxc1T was 31.7 mol%. Combining the results above, it was ascertained that strain Lxc1T represented a novel species of the genus Lacinutrix, for which the name Lacinutrix gracilariae sp. nov. is proposed. The type strain is Lxc1T ( = MCCC 1A01567T = KCTC 42808T).