| Literature DB >> 26553081 |
Bagher Larijani1, Hamidreza Aghayan2, Parisa Goodarzi3, Fereshteh Mohamadi-Jahani4, Abbas Norouzi-Javidan5, Ahmad Reza Dehpour6, Khadijeh Fallahzadeh2, Forough Azam Sayahpour7, Kazem Bidaki4, Babak Arjmand8.
Abstract
In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors' age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.Entities:
Keywords: Adipose-derived stem cell; Cell therapy; Good manufacturing practice; Mesenchymal stem cells
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Year: 2015 PMID： 26553081
Source DB: PubMed Journal: Acta Med Iran ISSN： 0044-6025