Literature DB >> 26552988

Heterologous Expression and Functional Characterization of the Exogenously Acquired Aminoglycoside Resistance Methyltransferases RmtD, RmtD2, and RmtG.

Laís L Corrêa1, Marta A Witek2, Natalia Zelinskaya2, Renata C Picão3, Graeme L Conn4.   

Abstract

The exogenously acquired 16S rRNA methyltransferases RmtD, RmtD2, and RmtG were cloned and heterologously expressed in Escherichia coli, and the recombinant proteins were purified to near homogeneity. Each methyltransferase conferred an aminoglycoside resistance profile consistent with m(7)G1405 modification, and this activity was confirmed by in vitro 30S methylation assays. Analyses of protein structure and interaction with S-adenosyl-l-methionine suggest that the molecular mechanisms of substrate recognition and catalysis are conserved across the 16S rRNA (m(7)G1405) methyltransferase family.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 26552988      PMCID： PMC4704232          DOI： 10.1128/AAC.02482-15

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  24 in total

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3.  Emergence of the 16S rRNA methylase RmtG in an extended-spectrum-β-lactamase-producing and colistin-resistant Klebsiella pneumoniae isolate in Chile.

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4.  MDR ST2179-CTX-M-15 Escherichia coli co-producing RmtD and AAC(6')-Ib-cr in a horse with extraintestinal infection, Brazil.

Authors:  Lucianne Leigue; José F G Warth; Luana C Melo; Ketrin C Silva; Rodrigo A Moura; Leandro Barbato; Lais C Silva; Ana C M Santos; Rosa M Silva; Nilton Lincopan
Journal:  J Antimicrob Chemother       Date:  2014-12-23       Impact factor: 5.790

5.  Coproduction of 16S rRNA methyltransferase RmtD or RmtG with KPC-2 and CTX-M group extended-spectrum β-lactamases in Klebsiella pneumoniae.

Authors:  Maria Fernanda C Bueno; Gabriela R Francisco; Jessica A O'Hara; Doroti de Oliveira Garcia; Yohei Doi
Journal:  Antimicrob Agents Chemother       Date:  2013-03-04       Impact factor: 5.191

6.  Klebsiella pneumoniae sequence type 11 isolate producing RmtG 16S rRNA methyltransferase from a patient in Miami, Florida.

Authors:  Fupin Hu; L Silvia Munoz-Price; Dennise DePascale; Jesabel I Rivera; Yohei Doi
Journal:  Antimicrob Agents Chemother       Date:  2014-05-19       Impact factor: 5.191

7.  Expansion of the aminoglycoside-resistance 16S rRNA (m(1)A1408) methyltransferase family: expression and functional characterization of four hypothetical enzymes of diverse bacterial origin.

Authors:  Marta A Witek; Graeme L Conn
Journal:  Biochim Biophys Acta       Date:  2014-06-22

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Review 9.  Aminoglycoside antibiotics in the 21st century.

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Journal:  BMC Genomics       Date:  2014-01-22       Impact factor: 3.969
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  2 in total

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Journal:  Curr Microbiol       Date:  2019-10-26       Impact factor: 2.188

2.  Functionally critical residues in the aminoglycoside resistance-associated methyltransferase RmtC play distinct roles in 30S substrate recognition.

Authors:  Meisam Nosrati; Debayan Dey; Atousa Mehrani; Sarah E Strassler; Natalia Zelinskaya; Eric D Hoffer; Scott M Stagg; Christine M Dunham; Graeme L Conn
Journal:  J Biol Chem       Date:  2019-10-08       Impact factor: 5.486
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