| Literature DB >> 26552679 |
Massimo Aureli1, Sara Grassi1, Sandro Sonnino1, Alessandro Prinetti2.
Abstract
The hypothesis that the Golgi apparatus is capable of sorting proteins and sending them to the plasma membrane through "lipid rafts," membrane lipid domains highly enriched in glycosphingolipids, sphingomyelin, ceramide, and cholesterol, was formulated by van Meer and Simons in 1988 and came to a turning point when it was suggested that lipid rafts could be isolated thanks to their resistance to solubilization by some detergents, namely Triton X-100. An incredible number of papers have described the composition and properties of detergent-resistant membrane fractions. However, the use of this method has also raised the fiercest criticisms. In this chapter, we would like to discuss the most relevant methodological aspects related to the preparation of detergent-resistant membrane fractions, and to discuss the importance of discriminating between what is present on a cell membrane and what we can prepare from cell membranes in a laboratory tube.Entities:
Keywords: Detergent-resistant membrane; Lipid raft; Liquid-ordered phase; Membrane domain; Microdomain; Sphingolipid
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Year: 2016 PMID： 26552679 DOI： 10.1007/978-1-4939-3170-5_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745