Xiao Qiao1, Xiaolong Tang2, Jianfu Zhang3, Ke Chen4, Yanming Zhang4, Changcheng Wang5, Sujuan Fei6, Jinzhou Zhu3, Shengping Zhu3, Zhangbo Liu3, Tingting Li3, Shengxiang Lv5, Yong Liang4. 1. Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China ; Department of Gastroenterology, The Affiliated Huai'an Hospital of Xuzhou Medical College 62 South Huaihai Road, Huai'an, 223002, Jiangsu, China. 2. Stem Cell Engineering Research Center, School of Medicine, Anhui University of Science & Technology Huainan 232001, China. 3. Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China. 4. Clinical Laboratory, The Affiliated Huai'an Hospital of Xuzhou Medical College Huai'an 223002, China. 5. Department of Gastroenterology, The Affiliated Huai'an Hospital of Xuzhou Medical College 62 South Huaihai Road, Huai'an, 223002, Jiangsu, China. 6. Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China.
Abstract
AIMS: We investigated the effffects and the possible mechanism of microinjection of histamine into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage (SGMD) in rats. The effect of microinjection of histamine into FN on SGMD was observed. METHODS: The model of SGMD was established by restraint and water (21 ± 1°C)-immersion (RWI) for 3 h in rats. The gastric mucosal damage index indicated the severity of SGMD. Western blotting was performed to assess gastric mucosal cell apoptosis and proliferation. RESULTS: We observed that histamine microinjection into the FN markedly attenuated SGMD in a dose-dependent manner, and was prevented by pre-treatment with the ranitidine (a selective histamine H2 receptor antagonist) into the FN. The effect of histamine was abolished by pre-treatment with 3-MPA (a glutamic acid decarboxylase antagonist) into the FN. There was a decrease in the discharge frequency of greater splanchnic nerve, and an increase in gastric mucosal blood flow after histamine injection into the FN. Additionally, anti-apoptotic and anti-oxidative factors of gastric mucosa might be involved in this process. CONCLUSION: The exogenous histamine in FN participates in the regulation of SGMD, and our results may help to provide new ideas on the treatment of gastroenterological diseases.
AIMS: We investigated the effffects and the possible mechanism of microinjection of histamine into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage (SGMD) in rats. The effect of microinjection of histamine into FN on SGMD was observed. METHODS: The model of SGMD was established by restraint and water (21 ± 1°C)-immersion (RWI) for 3 h in rats. The gastric mucosal damage index indicated the severity of SGMD. Western blotting was performed to assess gastric mucosal cell apoptosis and proliferation. RESULTS: We observed that histamine microinjection into the FN markedly attenuated SGMD in a dose-dependent manner, and was prevented by pre-treatment with the ranitidine (a selective histamine H2 receptor antagonist) into the FN. The effect of histamine was abolished by pre-treatment with 3-MPA (a glutamic acid decarboxylase antagonist) into the FN. There was a decrease in the discharge frequency of greater splanchnic nerve, and an increase in gastric mucosal blood flow after histamine injection into the FN. Additionally, anti-apoptotic and anti-oxidative factors of gastric mucosa might be involved in this process. CONCLUSION: The exogenous histamine in FN participates in the regulation of SGMD, and our results may help to provide new ideas on the treatment of gastroenterological diseases.
Entities:
Keywords:
Histamine; decussation of superior cerebellar peduncle; fastigial nucleus; lateral hypothalamic area; stress-induced gastric mucosal damage