Mingzhu Lu1, Jing Zhu2, Yang Ling2, Wenping Shi3, Changsong Zhang2, Haorong Wu4. 1. Department of The General Surgery, The Second Affiliated Hospital of Soochow University Suzhou 215004, China ; Department of The Hepatobiliary Surgery, Changzhou Cancer Hospital of Soochow University Changzhou 213002, China. 2. Clinical Oncology Laboratory, Changzhou Cancer Hospital of Soochow University Changzhou 213002, China. 3. Department of The Hepatobiliary Surgery, Changzhou Cancer Hospital of Soochow University Changzhou 213002, China. 4. Department of The General Surgery, The Second Affiliated Hospital of Soochow University Suzhou 215004, China.
Abstract
AIMS: Expression of gonadotropin-releasing hormone receptor (GnRHR) has been demonstrated in a number of malignancies. The aim is to investigate the expression of GnRHR and prognosis in gastric cancer. METHODS AND MATERIALS: GnRHR mRNA was examined in tumor and non-tumor tissues from 48 gastric cancer patients by Real-time PCR. The GnRHR protein expression was performed by immunohistochemical analysis. RESULTS: The expression of GnRHR mRNA was higher (mean ± SD, -10.06 ± 1.28) in gastric tumor tissues than matched non-tumor tissues (mean ± SD, -12.43 ± 1.33). GnRHR mRNA expression was associated with lymph node metastasis, distant metastasis, and TNM stage. We found the decreased expression of GnRHR mRNA were significantly correlated with poor overall survival (P = 0.003). Immunocytochemical staining of GnRHR in tumor tissues showed mainly weak staining (43.48%, 10/23) and moderate staining (21.74%, 5/23) in high GnRHR mRNA patients, and mainly negative staining in low GnRHR mRNA patients. And the staining of GnRHR was not detection in tumor tissues for more than half of gastric patients (52.08%, 25/48). These results implied that the loss of GnRHR protein could be a main event in gastric cancer. CONCLUSION: The GnRHR expression is very low in gastric cancer, and the loss of GnRHR expression could be a poor prognostic factor, which implied that GnRHR could play an important role in the development of gastric cancer.
AIMS: Expression of gonadotropin-releasing hormone receptor (GnRHR) has been demonstrated in a number of malignancies. The aim is to investigate the expression of GnRHR and prognosis in gastric cancer. METHODS AND MATERIALS: GnRHR mRNA was examined in tumor and non-tumor tissues from 48 gastric cancerpatients by Real-time PCR. The GnRHR protein expression was performed by immunohistochemical analysis. RESULTS: The expression of GnRHR mRNA was higher (mean ± SD, -10.06 ± 1.28) in gastric tumor tissues than matched non-tumor tissues (mean ± SD, -12.43 ± 1.33). GnRHR mRNA expression was associated with lymph node metastasis, distant metastasis, and TNM stage. We found the decreased expression of GnRHR mRNA were significantly correlated with poor overall survival (P = 0.003). Immunocytochemical staining of GnRHR in tumor tissues showed mainly weak staining (43.48%, 10/23) and moderate staining (21.74%, 5/23) in high GnRHR mRNA patients, and mainly negative staining in low GnRHR mRNA patients. And the staining of GnRHR was not detection in tumor tissues for more than half of gastricpatients (52.08%, 25/48). These results implied that the loss of GnRHR protein could be a main event in gastric cancer. CONCLUSION: The GnRHR expression is very low in gastric cancer, and the loss of GnRHR expression could be a poor prognostic factor, which implied that GnRHR could play an important role in the development of gastric cancer.
Authors: Hanna Pincas; Soon Gang Choi; Qian Wang; Jingjing Jia; Judith L Turgeon; Stuart C Sealfon Journal: Mol Cell Endocrinol Date: 2013-08-28 Impact factor: 4.102