| Literature DB >> 26550217 |
Yuxiang Fan1, Abulimiti Patima1, Yu Chen2, Fanye Zeng1, Wenting He1, Lingjuan Luo1, Yanghua Jie1, Yanhua Zhu1, Liping Zhang1, Jun Lei1, Xinmei Xie1, Hongliang Zhang1.
Abstract
To investigate the cytotoxic effects of β-carboline alkaloids on human gastric cancer SGC-7901 cells. Human gastric cancer SGC-790s1 cells were treated with β-carboline alkaloids at the concentration of 0, 10, 20, 30 and 40 μg/ml for 48 hr. Cell viability was measured by Cell Counting Kit-8 assay. Cell apoptosis was detected by Hoechst 33258 staining and DNA fragmentation analysis. The expression of phosphatase and tensin homolog (PTEN) and extracellular signal-regulated kinase (ERK) was examined by quantitative real-time PCR (qRT-PCR) assay and western blot analysis. β-carboline alkaloids inhibited the growth of SGC-7901 cells concentration dependently. β-carboline alkaloids treated SGC-7901 cells displayed apoptotic nuclei as detected using Hoechst 33258 staining. β-carboline alkaloids also induced DNA ladder, indicative of apoptosis in SGC-7901 cells concentration-dependently. Furthermore, β-carboline alkaloids increased PTEN and decreased ERK mRNA expression in SGC-7901 cells in a concentration dependent manner. They also increased PTEN and decreased ERK protein expression. β-carboline alkaloids inhibit the growth and induce apoptosis of SGC-7901 cells. The cytotoxic effects of β-carboline alkaloids might correlate with increased PTEN expression and decreased ERK expression in SGC-7901 cells.Entities:
Keywords: ERK; PTEN; apoptosis; human SGC-7901 cells; β-carboline alkaloids
Year: 2015 PMID： 26550217 PMCID： PMC4612902
Source DB: PubMed Journal: Int J Clin Exp Med ISSN： 1940-5901