Role of WISP3 siRNA in proliferation, apoptosis and invasion of bladder cancer cells.

Bladder cancer (BC) is the most common cancers of the urinary tract worldwide, killing thousands of people a year. WISP3 is a cysteine-rich protein that belongs to the CCN (Cyr61, CTGF, Nov) family of proteins. Increasing evidences have linked abnormal levels of CCN family members to tumorigenic effects. In the present study, we found that WISP3 was overexpressed in BC. Knockdown of WISP3by RNA interference in two BC cell lines (5367 and SCaBER cells) significantly inhibited cell proliferation, which may be mediated by cell cycle arrest in G1 phase. Moreover, silencing of WISP3 also induced cell apoptosis via increasing the expression of caspase 3 and caspase 9. Depletion of WISP3 notably inhibited the invasion of BC cells. Our data suggests that inhibition of WISP3 may be a therapeutic strategy for BC.