Raziye Akcılar1, Sezer Akçer2, Hasan Şimşek1, Aydın Akcılar3, Zeynep Bayat4, Osman Genç1. 1. Department of Physiology, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey. 2. Department of Anatomy, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey. 3. Experimental Animal Research Center, Faculty of Medicine, University of Dumlupınar Kütahya, Turkey. 4. Department of Biochemistry, Faculty of Arts and Sciences, University of Dumlupınar Kütahya, Turkey.
Abstract
BACKGROUND: Hypertension is a risk factor for the cardiovascular diseases. Ozone as a therapeutic agent for the treatment of several disorders. We aimed to observe the effects of ozone on the blood pressure in DOCA-salt hypertensive rats. METHODS: Twenty three young Sprague Dawley male rats were divided into three groups; Control (C), Hypertension (H) and Hypertension + Ozone (HO). Hypertension was induced by injection of DOCA-salt (25 mg/kg, s.c.) twice weekly, 4 weeks, whereas intraperitoneal ozone was administered (1.1 mg/kg) for 10 days. Serum endothelin-1, nitric oxide and renin levels were measured with ELISA. Blood pressures were monitored using a tail cuff system. Endothelin-1, ET receptor A and ET receptor B mRNA expression in heart and vascular tissue were assessed by quantitative reverse transcription polymerase chain reaction. RESULTS: Blood pressure, serum endothelin-1 and ET receptor A mRNA expression levels were increased in H group, whereas serum renin, nitric oxide and ET receptor B mRNA expression levels in the heart and vascular tissue decreased compared with C and HO groups, which were counteracted by ozone treatment. CONCLUSION: Ozone treatment decreases blood pressure and is effective in preventing the progression of hypertensive disease, the mechanisms of which are associated with anti-vasoconstrictor effects through reducing the levels of serum endothelin-1 and ET receptor A mRNA expression in the heart and vascular tissue.
BACKGROUND:Hypertension is a risk factor for the cardiovascular diseases. Ozone as a therapeutic agent for the treatment of several disorders. We aimed to observe the effects of ozone on the blood pressure in DOCA-salthypertensiverats. METHODS: Twenty three young Sprague Dawley male rats were divided into three groups; Control (C), Hypertension (H) and Hypertension + Ozone (HO). Hypertension was induced by injection of DOCA-salt (25 mg/kg, s.c.) twice weekly, 4 weeks, whereas intraperitoneal ozone was administered (1.1 mg/kg) for 10 days. Serum endothelin-1, nitric oxide and renin levels were measured with ELISA. Blood pressures were monitored using a tail cuff system. Endothelin-1, ET receptor A and ET receptor B mRNA expression in heart and vascular tissue were assessed by quantitative reverse transcription polymerase chain reaction. RESULTS: Blood pressure, serum endothelin-1 and ET receptor A mRNA expression levels were increased in H group, whereas serum renin, nitric oxide and ET receptor B mRNA expression levels in the heart and vascular tissue decreased compared with C and HO groups, which were counteracted by ozone treatment. CONCLUSION:Ozone treatment decreases blood pressure and is effective in preventing the progression of hypertensive disease, the mechanisms of which are associated with anti-vasoconstrictor effects through reducing the levels of serum endothelin-1 and ET receptor A mRNA expression in the heart and vascular tissue.
Authors: Urmila P Kodavanti; Ronald Thomas; Allen D Ledbetter; Mette C Schladweiler; Jonathan H Shannahan; J Grace Wallenborn; Amie K Lund; Matthew J Campen; Elizabeth O Butler; Reddy R Gottipolu; Abraham Nyska; Judy E Richards; Deborah Andrews; Richard H Jaskot; John McKee; Sainath R Kotha; Rishi B Patel; Narasimham L Parinandi Journal: Environ Health Perspect Date: 2010-10-27 Impact factor: 9.031
Authors: Marion Ouidir; Fasil Tekola-Ayele; Timothy Canty; Katherine L Grantz; Anthony Sciscione; Daniel Tong; Rena R Jones; Rajeshwari Sundaram; Andrew Williams; Danielle Stevens; Pauline Mendola Journal: Environ Res Date: 2021-07-21 Impact factor: 6.498