| Literature DB >> 26549461 |
Lothar C Dieterich1, Sarah Klein1, Anthony Mathelier2, Adriana Sliwa-Primorac1, Qiaoli Ma1, Young-Kwon Hong3, Jay W Shin4, Michito Hamada5, Marina Lizio4, Masayoshi Itoh4, Hideya Kawaji4, Timo Lassmann6, Carsten O Daub4, Erik Arner4, Piero Carninci4, Yoshihide Hayashizaki7, Alistair R R Forrest4, Wyeth W Wasserman2, Michael Detmar8.
Abstract
VEGF-C/VEGFR-3 signaling plays a central role in lymphatic development, regulating the budding of lymphatic progenitor cells from embryonic veins and maintaining the expression of PROX1 during later developmental stages. However, how VEGFR-3 activation translates into target gene expression is still not completely understood. We used cap analysis of gene expression (CAGE) RNA sequencing to characterize the transcriptional changes invoked by VEGF-C in LECs and to identify the transcription factors (TFs) involved. We found that MAFB, a TF involved in differentiation of various cell types, is rapidly induced and activated by VEGF-C. MAFB induced expression of PROX1 as well as other TFs and markers of differentiated LECs, indicating a role in the maintenance of the mature LEC phenotype. Correspondingly, E14.5 Mafb(-/-) embryos showed impaired lymphatic patterning in the skin. This suggests that MAFB is an important TF involved in lymphangiogenesis.Entities:
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Year: 2015 PMID： 26549461 DOI： 10.1016/j.celrep.2015.10.002
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423