Juliana Mara Serpeloni1, Ana Flávia Leal Specian2, Diego Luis Ribeiro2, Katiuska Tuttis2, Wagner Vilegas3, Wilner Martínez-López4, Anne Lígia Dokkedal5, Luiz Leonardo Saldanha6, Ilce Mara de Syllos Cólus2, Eliana Aparecida Varanda7. 1. Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil. Electronic address: julianaserpeloni@yahoo.com.br. 2. Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina 86057-970, Brazil. 3. Experimental Campus of São Vicente, São Paulo State University (UNESP), São Vicente 11350-000, Brazil. 4. Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay. 5. Department of Biological Sciences, Faculty of Sciences, São Paulo State University (UNESP), Bauru 17033-360, Brazil. 6. Department of Biological Sciences, Faculty of Sciences, São Paulo State University (UNESP), Bauru 17033-360, Brazil; Department of Botany, Institute of Bioscience, São Paulo State University (UNESP), Botucatu 18618-970, Brazil. 7. Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The Brazilian "Cerrado" is an important source of natural products, such as Myrcia bella Cambess (MB, also known as "mercurinho"). MB leaves are popularly used for the treatment of diabetes and gastrointestinal disorders; however, only its hypoglycemic activity has been experimentally described. AIM OF THE STUDY: Because MB is used to treat gastrointestinal disorders, the present study characterized biological activities of hydroalcoholic MB extract in human normal and tumor gastric cells. MATERIALS AND METHODS: Cytotoxic, antiproliferative, genotoxic and protective effects were evaluated, as well as the effects of the MB extract on gene expression. RESULTS: The MB extract induced cytotoxicity in tumor cells at lower concentrations compared with normal cells as assessed by the MTT assay. Moreover, the MB extract induced necrosis based on acridine orange/ethidium bromide staining. An antiproliferative effect was evidenced through an arrest in the G2/M phase detected by flow cytometry and a decrease in the nuclear division index using the cytokinesis-block micronucleus cytome assay. Cells treated with MB extract combined with doxorubicin (DXR) showed increased NUBDs, which may be related to the gene amplification of CCND1. Antimutagenic effects were also observed and may be associated with the antioxidant activities detected using the CM-H2DCFDA probe. CONCLUSIONS: Our findings showed the following: (a) high concentrations of MB induced cytotoxicity and cell death by necrosis; (b) its antiproliferative effect was associated with G2/M arrest; and (c) its antioxidant activity could be responsible for the observed antimutagenic effects and for protective effects against gastrointestinal disorders previously described to MB. Although these effects are not specific to normal or tumor cells, they provide a panel of biological activities for further exploration.
ETHNOPHARMACOLOGICAL RELEVANCE: The Brazilian "Cerrado" is an important source of natural products, such as Myrcia bella Cambess (MB, also known as "mercurinho"). MB leaves are popularly used for the treatment of diabetes and gastrointestinal disorders; however, only its hypoglycemic activity has been experimentally described. AIM OF THE STUDY: Because MB is used to treat gastrointestinal disorders, the present study characterized biological activities of hydroalcoholic MB extract in human normal and tumor gastric cells. MATERIALS AND METHODS:Cytotoxic, antiproliferative, genotoxic and protective effects were evaluated, as well as the effects of the MB extract on gene expression. RESULTS: The MB extract induced cytotoxicity in tumor cells at lower concentrations compared with normal cells as assessed by the MTT assay. Moreover, the MB extract induced necrosis based on acridine orange/ethidium bromide staining. An antiproliferative effect was evidenced through an arrest in the G2/M phase detected by flow cytometry and a decrease in the nuclear division index using the cytokinesis-block micronucleus cytome assay. Cells treated with MB extract combined with doxorubicin (DXR) showed increased NUBDs, which may be related to the gene amplification of CCND1. Antimutagenic effects were also observed and may be associated with the antioxidant activities detected using the CM-H2DCFDA probe. CONCLUSIONS: Our findings showed the following: (a) high concentrations of MB induced cytotoxicity and cell death by necrosis; (b) its antiproliferative effect was associated with G2/M arrest; and (c) its antioxidant activity could be responsible for the observed antimutagenic effects and for protective effects against gastrointestinal disorders previously described to MB. Although these effects are not specific to normal or tumor cells, they provide a panel of biological activities for further exploration.
Authors: Ana Flávia L Specian; Juliana M Serpeloni; Katiuska Tuttis; Diego L Ribeiro; Heloísa L Cilião; Eliana A Varanda; Miriam Sannomiya; Wilner Martinez-Lopez; Wagner Vilegas; Ilce M S Cólus Journal: Cytotechnology Date: 2016-06-25 Impact factor: 2.058
Authors: Jaqueline Ferreira Campos; David Tsuyoshi Hiramatsu de Castro; Marcio José Damião; Heron F Vieira Torquato; Edgar J Paredes-Gamero; Carlos Alexandre Carollo; Leticia M Estevinho; Kely de Picoli Souza; Edson Lucas Dos Santos Journal: Oxid Med Cell Longev Date: 2016-10-10 Impact factor: 6.543
Authors: Catarina Dos Santos; Renan S Galaverna; Celio F F Angolini; Vania V A Nunes; Luiz F R de Almeida; Ana L T G Ruiz; João E de Carvalho; Regina M T Duarte; Marta C T Duarte; Marcos N Eberlin Journal: Molecules Date: 2018-04-24 Impact factor: 4.411