Min Zha1, Wei Xu2, Peter M Jones3, Zilin Sun4. 1. Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, School of medicine, Southeast University, Nanjing, China; Department of Endocrinology, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Hanzhong Road, Nanjing, China. 2. Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, School of medicine, Southeast University, Nanjing, China. 3. Diabetes Research Group, Division of Diabetes and Nutritional Sciences, Faculty of Life Sciences and Medicine, King's College London, London SE1 1UL, UK. 4. Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, School of medicine, Southeast University, Nanjing, China. Electronic address: sunzilin1963@126.com.
Abstract
BACKGROUND AND AIMS: We have previously demonstrated that islet stellate cells (ISCs) exhibiting a similar phenotype to classical pancreatic stellate cells (PSCs) could be isolated from rat islets, where they may contribute to islet fibrosis in type 2 diabetes mellitus (T2DM). This study was designed to determine whether human islets also contain ISC. MATERIALS AND METHODS: Using standard explants techniques, human ISCs were enriched from freshly isolated human islets. Immunofluorescence visualization of markers for PSCs(α-smooth muscle actin;α-SMA), desmin, vimentin, glial fibrillary acidic protein (GFAP) was used to characterize the human ISC. Cell counting kit-8 (CCK-8) was used to assess the proliferation of ISC. The wound-healing assay and the transwell migration were used to assess the migration capacity of ISC. Immunofluorescence against collagen typesI (col-I), collagen typesIII (col-III) and fibronectin (FN) was performed to identify extracellular matrix (ECM) component synthesized by ISC. Adipogenic and osteogenic differentiation were tried to detected stem cell potential. RESULTS: In culture, ISC with triangular shape grow out from human islets. The passaged ISC expressed α-SMA, desmin, vimentin, GFAP and was positive for col-I, col-III and FN. The proliferation and migration ability of ISC was significantly slower than those of PSC. And both the human PSC and ISC were able to differentiate in vitro into adipocyte- and osteoblast-like cells. CONCLUSION: Similar to our previous rat experiment, the current study shows that human islets also contain ISC which is phenotypically similar but not identical to human PSC.
BACKGROUND AND AIMS: We have previously demonstrated that islet stellate cells (ISCs) exhibiting a similar phenotype to classical pancreatic stellate cells (PSCs) could be isolated from rat islets, where they may contribute to islet fibrosis in type 2 diabetes mellitus (T2DM). This study was designed to determine whether human islets also contain ISC. MATERIALS AND METHODS: Using standard explants techniques, human ISCs were enriched from freshly isolated human islets. Immunofluorescence visualization of markers for PSCs(α-smooth muscle actin;α-SMA), desmin, vimentin, glial fibrillary acidic protein (GFAP) was used to characterize the human ISC. Cell counting kit-8 (CCK-8) was used to assess the proliferation of ISC. The wound-healing assay and the transwell migration were used to assess the migration capacity of ISC. Immunofluorescence against collagen typesI (col-I), collagen typesIII (col-III) and fibronectin (FN) was performed to identify extracellular matrix (ECM) component synthesized by ISC. Adipogenic and osteogenic differentiation were tried to detected stem cell potential. RESULTS: In culture, ISC with triangular shape grow out from human islets. The passaged ISC expressed α-SMA, desmin, vimentin, GFAP and was positive for col-I, col-III and FN. The proliferation and migration ability of ISC was significantly slower than those of PSC. And both the human PSC and ISC were able to differentiate in vitro into adipocyte- and osteoblast-like cells. CONCLUSION: Similar to our previous rat experiment, the current study shows that human islets also contain ISC which is phenotypically similar but not identical to human PSC.
Authors: Y X Li; Y Q Sang; Yan Sun; X K Liu; H F Geng; Min Zha; Ben Wang; Fei Teng; H J Sun; Yu Wang; Q Q Qiu; Xiu Zang; Yun Wang; T T Wu; Peter M Jones; Jun Liang; Wei Xu Journal: Int J Med Sci Date: 2020-07-02 Impact factor: 3.738
Authors: Wei Xu; Jun Liang; H F Geng; Jun Lu; Rui Li; X L Wang; Qian Lv; Ying Liu; Jie Wang; X K Liu; Peter M Jones; Zl Sun Journal: Int J Endocrinol Date: 2019-04-11 Impact factor: 3.257
Authors: Wei Xu; HouFa Geng; Jun Liang; Ying Liu; Qian Lv; Jie Wang; Rui Li; Xiu Li Wang; Xui Kui Liu; Peter M Jones; Zi Lin Sun Journal: J Diabetes Investig Date: 2019-08-20 Impact factor: 4.232