| Literature DB >> 26545323 |
Yongfeng Cheng1, Haiming Wei2, Rui Sun2, Zhigang Tian2, Xiaodong Zheng3.
Abstract
Bradford assay is one of the most common methods for measuring protein concentrations. However, some pharmaceutical excipients, such as detergents, interfere with Bradford assay even at low concentrations. Protein precipitation can be used to overcome sample incompatibility with protein quantitation. But the rate of protein recovery caused by acetone precipitation is only about 70%. In this study, we found that sucrose not only could increase the rate of protein recovery after 1 h acetone precipitation, but also did not interfere with Bradford assay. So we developed a method for rapid protein quantitation in protein drugs even if they contained interfering substances.Entities:
Keywords: Bradford assay; Protein precipitation; Sucrose
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Year: 2015 PMID： 26545323 DOI： 10.1016/j.ab.2015.10.013
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365