Correction: Defining the Roles of IFN-γ and IL-17A in Inflammation and Protection against Helicobacter pylori Infection.

Fig 5 is incorrect, and there are a number of errors in the caption for Fig 5. Please see the corrected Fig 5 and its caption here.

Fig 5

Neutralization of IL-17A abrogates protection, and reduces gastric inflammation and proliferation of MLN cells in sublingually immunized IFN-γ-/- mice.

IFN-γ-/- mice were sublingually immunized with H. pylori lysate antigens and CT (SL) or left unimmunized (Inf) and infected with live H. pylori bacteria. Mice were injected intraperitoneally neutralizing IL-17A antibody (αIL-17A) or control IgG antibody (IgG). Two weeks post infection mice were sacrificed. A. Stomach tissue was analyzed for H. pylori colonization by quantitative culture and expressed as mean log10 cfu per stomach, and SEM. B. analysis of IL-17A secretion in stomach tissue extracts and C. Atrophy and D. Infiltration in stomach tissue was scored. n = 6–11 mice/group, pool of two experiments. Bars represent mean. Statistically significant difference between sublingually immunized IFN-γ-/- mice injected neutralizing IL-17A antibody compared to immunized mice injected isotype control antibody was calculated by an unpaired two-tailed t-test with Welch correction and denoted by * (p<0.05), ** (p<0.01), *** (p<0.001). E. single cell suspensions of MLN were prepared and cultured in vitro with H. pylori lysate antigens. Counts per minute (cpm) of incorporated radioactive thymidine was used as a measure of proliferation of the cells. Bars represent mean value and standard deviation (SD) counts in 6 individual wells in pooled mice (n = 5–7 mice/group) F. Supernatants were collected from in vitro cultured MLN (from E) and assessed for IL-17A shown in pg/ml, of six pooled wells. Data pool of two independent experiments. G. Stomach tissue was analyzed for gene expression of Lcn (Lipocalin-2) and expressed as relative gene expression where unimmunized infection control was set to 1. Statistically significant difference between sublingually immunized IFN-γ-/- mice injected neutralizing IL-17A antibody compared to immunized mice injected isotype control antibody was calculated by an unpaired two-tailed t-test with Welch's correction and denoted by *** (p<0.001).