| Literature DB >> 26542719 |
Mei Ling Leong1, Evan W Newell2.
Abstract
Mass cytometry is flow cytometry based on single cell mass spectrometry with decreased crosstalk between channels and an ability to probe >40 parameters per cell, making it well suited for multiplexed assays. Peptide major histocompatibility (MHC) tetramer staining allows direct detection of antigen specific cells and is also amenable to multiplexing/combinatorial approaches. Here we describe methods for multiplexed pMHC-tetramer staining using mass cytometry.Entities:
Keywords: Antigen-specific T cell; Combinatorial tetramer staining; Mass cytometry; Peptide-MHC tetramer
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Year: 2015 PMID： 26542719 DOI： 10.1007/978-1-4939-2987-0_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745