| Literature DB >> 26542531 |
Hebin Liao1,2,3, Mafeng Liu4,5,6, Xingjun Cheng1,2,3, Dekang Zhu1,2,3, Mingshu Wang1,2,3, Renyong Jia1,2,3, Shun Chen1,2,3, Kunfeng Sun1,2,3, Qiao Yang1,2,3, Francis Biville7, Anchun Cheng8,9,10.
Abstract
Riemerella anatipestifer (R. anatipestifer) is among the most prevalent duck pathogens, causing acute or chronic septicemia characterized by serositis. Riemerella anatipestifer can be grown on blood-enriched media, in vitro, which provides a hemin source essential for the sustainment of R. anatipestifer and activation of hemin-uptake systems. However, the genes associated with hemin uptake cannot be identified exclusively through genome sequence analysis. Here, we show that R. anatipestifer encodes outer-membrane hemin-binding proteins. Hemin-binding proteins were identified in the cytoplasm with apparent molecular mass of ~45/37/33/23/20/13 kDa, and outer membrane with apparent molecular mass of ~90/70/60/50/15 kDa by batch affinity chromatography and hemin-blotting assays. Our results indicate that these proteins are involved in hemin acquisition. Finally, hemin-binding assay further showed that R. anatipestifer can bind hemin and this capability is increased in iron limited medium, indicating the hemin-uptake system of R. anatipestifer was regulated by iron.Entities:
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Year: 2015 PMID： 26542531 DOI： 10.1007/s00284-015-0932-5
Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188