Richard Colling1, Lai Mun Wang1, Elizabeth Soilleux2. 1. Department of Cellular Pathology, Oxford University Hospitals NHS Trust, Oxford, UK. 2. Department of Cellular Pathology, Oxford University Hospitals NHS Trust, Oxford, UK Nuffield Division of Clinical Laboratory Sciences, University of Oxford, Oxford, UK.
Abstract
BACKGROUND: Testing for BRAF mutations in colorectal carcinoma (CRC) is important in the screening pathway for Lynch syndrome and is of prognostic value to guide management. This is a diagnostic accuracy study of the Idylla system, a novel and automated alternative PCR system. METHODS: 100 consecutive formalin-fixed, paraffin-embedded CRC resection cases were tested for BRAF mutations using the Idylla automated platform and compared with standard (Cobas) PCR. RESULTS: The sensitivity of the Idylla BRAF test was 100% and the specificity was 96%. Only one discordant Idylla positive/standard PCR negative result occurred and on Droplet Digital PCR demonstrated a mutation not identified by traditional PCR in this case. CONCLUSION: This study has validated the Idylla system for BRAF testing in CRC and demonstrated a possibly greater sensitivity, in addition to cost effectiveness and shorter turnaround time, when compared with standard PCR. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/
BACKGROUND: Testing for BRAF mutations in colorectal carcinoma (CRC) is important in the screening pathway for Lynch syndrome and is of prognostic value to guide management. This is a diagnostic accuracy study of the Idylla system, a novel and automated alternative PCR system. METHODS: 100 consecutive formalin-fixed, paraffin-embedded CRC resection cases were tested for BRAF mutations using the Idylla automated platform and compared with standard (Cobas) PCR. RESULTS: The sensitivity of the Idylla BRAF test was 100% and the specificity was 96%. Only one discordant Idylla positive/standard PCR negative result occurred and on Droplet Digital PCR demonstrated a mutation not identified by traditional PCR in this case. CONCLUSION: This study has validated the Idylla system for BRAF testing in CRC and demonstrated a possibly greater sensitivity, in addition to cost effectiveness and shorter turnaround time, when compared with standard PCR. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/
Authors: Cornelis Bisschop; Arja Ter Elst; Lisette J Bosman; Inge Platteel; Mathilde Jalving; Anke van den Berg; Arjan Diepstra; Bettien van Hemel; Gilles F H Diercks; Geke A P Hospers; Ed Schuuring Journal: Melanoma Res Date: 2018-04 Impact factor: 3.599
Authors: Louise Johnston; Michael Power; Philip Sloan; Anna Long; Angela Silmon; Ben Chaffey; Andrea Jane Lisgo; Liam Little; Ellen Vercauteren; Torben Steiniche; Tine Meyer; John Simpson Journal: J Clin Pathol Date: 2017-09-12 Impact factor: 3.411