Vincent Thomas de Montpréville1, Pauline Quilhot2, Lara Chalabreysse3, Anne De Muret4, Véronique Hofman5, Sylvie Lantuéjoul6, Marie Parrens7, Marie-José Payan8, Isabelle Rouquette9, Véronique Secq8, Nicolas Girard10, Benjamin Besse11, Alexander Marx12, Thierry Jo Molina13. 1. Centre Chirurgical Marie Lannelongue, Département de Pathologie, Le Plessis Robinson, France. Electronic address: v.thomasdemontprevil@ccml.fr. 2. Centre Chirurgical Marie Lannelongue, Département de Pathologie, Le Plessis Robinson, France. 3. Hôpital Louis-Pradel, Département de Pathologie, Lyon, France. 4. Chu de Tours, Département de Pathologie, Tours, France. 5. Hôpital Pasteur, CHU de Nice, Laboratoire de Pathologie Clinique et Expérimentale, Nice, France. 6. CHU de Grenoble, Département d'Anatomie et de Cytologie Pathologiques, Grenoble, France. 7. CHU de Bordeaux, Dr Parrens: EA 2406 Département de Pathologie, Bordeaux, France. 8. Hôpital Nord, APHM, Laboratoire d'Anatomie Pathologique, Marseille, France. 9. CHU Rangueil, Service d'Anatomie Pathologique, Toulouse, France. 10. Hôpital Louis-Pradel, Hospices Civils de Lyon, Département des Maladies Respiratoires, Lyon, France. 11. Institut Gustave Roussy, Département de Médecine, Villejuif, France. 12. Centre Universitaire Médical de Mannheim, Université de Heidelberg, Institut de Pathologie, Mannheim, Germany. 13. Université Paris Descartes, Sorbonne Paris Cité, Service de Pathologie, AP-HP Hôpital Necker Enfants Malades, Paris, France.
Abstract
OBJECTIVES: Glucose-transporter-1 (Glut-1) may be a useful marker for differentiating B3 thymomas and thymic carcinomas. Since the literature is limited, we undertook a study to evaluate its diagnostic value in a series of thymic epithelial tumors. MATERIALS AND METHODS: Glut-1 expression was studied by the group of pathologists linked to the French national oncological network RYTHMIC. Immunostaining was performed on a whole section of one paraffin block in a series of 92 successive surgical specimens. Patterns (focal, zonal, diffuse) and intensity of Glut-1 expression were assessed and compared with WHO histological subtypes. RESULTS: Expression was mainly restricted to epithelial cells. Immature T-lymphocytes were negative. A diffuse, moderate or strong staining was observed in most thymic carcinomas (15/16). In B3 thymomas (10/11) and in B3 thymomas borderline to thymic carcinomas (5/6), a moderate to strong zonal staining was observed at distance from vessels and fibrous septa. This pattern sometimes created the aspect of an anastomosing network in large cellular lobules. In B1 thymomas, immunostaining highlighted foci of medullary differentiation (7/8). B2 thymomas (n=25) were heterogeneous, with a spectrum of patterns ranging between those of B1 and B3 thymomas. Type A thymomas (n=5) mostly presented a weak positivity but one aggressive case showed zonal moderate/strong positivity. Most AB thymomas (15/17) showed weak to moderate immunostaining in spindle cell areas. In micronodular thymomas (n=3), epithelial cells and B-lymphocytes were weakly positive while follicular dendritic cells were strongly highlighted. One metaplastic thymoma displayed diffuse and moderate positivity. CONCLUSION: Glut-1 expression globally depended on histological subtypes and the staining patterns (diffuse or zonal) were different between thymic carcinomas and type B3 thymomas. A comparative study of Glut-1 expression in atypical versus conventional type A thymomas appears warranted. Otherwise, restriction to epithelial cells makes likely a correlation with clinical assessment of glucose uptake in lymphocyte-poor tumors.
OBJECTIVES:Glucose-transporter-1 (Glut-1) may be a useful marker for differentiating B3 thymomas and thymic carcinomas. Since the literature is limited, we undertook a study to evaluate its diagnostic value in a series of thymic epithelial tumors. MATERIALS AND METHODS:Glut-1 expression was studied by the group of pathologists linked to the French national oncological network RYTHMIC. Immunostaining was performed on a whole section of one paraffin block in a series of 92 successive surgical specimens. Patterns (focal, zonal, diffuse) and intensity of Glut-1 expression were assessed and compared with WHO histological subtypes. RESULTS: Expression was mainly restricted to epithelial cells. Immature T-lymphocytes were negative. A diffuse, moderate or strong staining was observed in most thymic carcinomas (15/16). In B3 thymomas (10/11) and in B3 thymomas borderline to thymic carcinomas (5/6), a moderate to strong zonal staining was observed at distance from vessels and fibrous septa. This pattern sometimes created the aspect of an anastomosing network in large cellular lobules. In B1 thymomas, immunostaining highlighted foci of medullary differentiation (7/8). B2 thymomas (n=25) were heterogeneous, with a spectrum of patterns ranging between those of B1 and B3thymomas. Type A thymomas (n=5) mostly presented a weak positivity but one aggressive case showed zonal moderate/strong positivity. Most AB thymomas (15/17) showed weak to moderate immunostaining in spindle cell areas. In micronodular thymomas (n=3), epithelial cells and B-lymphocytes were weakly positive while follicular dendritic cells were strongly highlighted. One metaplastic thymoma displayed diffuse and moderate positivity. CONCLUSION:Glut-1 expression globally depended on histological subtypes and the staining patterns (diffuse or zonal) were different between thymic carcinomas and type B3 thymomas. A comparative study of Glut-1 expression in atypical versus conventional type A thymomas appears warranted. Otherwise, restriction to epithelial cells makes likely a correlation with clinical assessment of glucose uptake in lymphocyte-poor tumors.
Authors: Mounika Angirekula; Sindy Y Chang; Sarah M Jenkins; Patricia T Greipp; William R Sukov; Randolph S Marks; Kenneth R Olivier; Stephen D Cassivi; Anja C Roden Journal: Cancers (Basel) Date: 2022-05-05 Impact factor: 6.575
Authors: Vincent Thomas de Montpreville; Audrey Mansuet-Lupo; Cécile Le Naoures; Lara Chalabreysse; Anne De Muret; Véronique Hofman; Isabelle Rouquette; Nicolas Piton; Romain Dubois; Jose Carlos Benitez; Nicolas Girard; Benjamin Besse; Alexander Marx; Thierry Jo Molina Journal: Virchows Arch Date: 2021-02-24 Impact factor: 4.064
Authors: Mohammad Alwahsh; Robert Knitsch; Rosemarie Marchan; Jörg Lambert; Christian Hoerner; Xiaonan Zhang; Berthold Schalke; De-Hyung Lee; Elena Bulut; Thomas Graeter; German Ott; Katrin S Kurz; Gerhard Preissler; Sebastian Schölch; Joviana Farhat; Zhihan Yao; Carsten Sticht; Philipp Ströbel; Roland Hergenröder; Alexander Marx; Djeda Belharazem Journal: Cancers (Basel) Date: 2022-03-18 Impact factor: 6.639