| Literature DB >> 2653102 |
R C Clough1, S R Barnum, J G Jaworski.
Abstract
The synthesis of high specific radioactivity [14C]-acetyl-Coenzyme A from [14C]sodium acetate, 2,6-dichlorobenzoic acid, 1,1'-carbonyldiimidazole, and CoA is reported. Starting with 1 mumol of [14C]sodium acetate, this method yields pure [14C]acetyl-CoA in yields approaching 40%. Chromatography on a reversed-phase ODS column was used to separate acetyl-CoA from Coenzyme A and side products. The acetylating agent is apparently a reaction intermediate, acetylimidazole.Entities:
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Year: 1989 PMID： 2653102 DOI： 10.1016/0003-2697(89)90276-5
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365