Monica Borghi1,2, De-Yu Xie3. 1. Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA. monica.borghi@wur.nl. 2. Laboratory of Plant Physiology, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands. monica.borghi@wur.nl. 3. Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA. dxie@ncsu.edu.
Abstract
MAIN CONCLUSION: Arabidopsis promoters of genes BANYULS and FRUITFULL are transcribed in Camelina. They triggered the transcription of limonene synthase and induced higher limonene production in seeds and fruits than CaMV 35S promoter. Camelina sativa (Camelina) is an oilseed crop of relevance for the production of biofuels and the plant has been target of a recent and intense program of genetic manipulation aimed to increase performance, seed yield and to modify the fatty acid composition of the oil. Here, we have explored the performance of two Arabidopsis thaliana (Arabidopsis) promoters in triggering transgene expression in Camelina. The promoters of two genes BANYULS (AtBAN pro ) and FRUITFULL (AtFUL pro ), which are expressed in seed coat and valves of Arabidopsis, respectively, have been chosen to induce the expression of limonene synthase (LS) from Citrus limon. In addition, the constitutive CaMV 35S promoter was utilized to overexpress LS in Camelina . The results of experiments revealed that AtBAN pro and AtFUL pro are actively transcribed in Camelina where they also retain specificity of expression in seeds and valves as previously observed in Arabidopsis. LS induced by AtBAN pro and AtFUL pro leads to higher limonene production in seeds and fruits than when the CaMV 35S was used to trigger the expression. In conclusion, the results of experiments indicate that AtBAN pro and AtFUL pro can be successfully utilized to induce the expression of the transgenes of interest in seeds and fruits of Camelina.
MAIN CONCLUSION:Arabidopsis promoters of genes BANYULS and FRUITFULL are transcribed in Camelina. They triggered the transcription of limonene synthase and induced higher limonene production in seeds and fruits than CaMV 35S promoter. Camelina sativa (Camelina) is an oilseed crop of relevance for the production of biofuels and the plant has been target of a recent and intense program of genetic manipulation aimed to increase performance, seed yield and to modify the fatty acid composition of the oil. Here, we have explored the performance of two Arabidopsis thaliana (Arabidopsis) promoters in triggering transgene expression in Camelina. The promoters of two genes BANYULS (AtBAN pro ) and FRUITFULL (AtFUL pro ), which are expressed in seed coat and valves of Arabidopsis, respectively, have been chosen to induce the expression of limonene synthase (LS) from Citrus limon. In addition, the constitutive CaMV 35S promoter was utilized to overexpress LS in Camelina . The results of experiments revealed that AtBAN pro and AtFUL pro are actively transcribed in Camelina where they also retain specificity of expression in seeds and valves as previously observed in Arabidopsis. LS induced by AtBAN pro and AtFUL pro leads to higher limonene production in seeds and fruits than when the CaMV 35S was used to trigger the expression. In conclusion, the results of experiments indicate that AtBAN pro and AtFUL pro can be successfully utilized to induce the expression of the transgenes of interest in seeds and fruits of Camelina.
Authors: Huu Tam Nguyen; Hyunwoo Park; Karen L Koster; Rebecca E Cahoon; Hanh T M Nguyen; John Shanklin; Thomas E Clemente; Edgar B Cahoon Journal: Plant Biotechnol J Date: 2014-07-26 Impact factor: 9.803
Authors: Michael Gutensohn; Irina Orlova; Thuong T H Nguyen; Rachel Davidovich-Rikanati; Mario G Ferruzzi; Yaron Sitrit; Efraim Lewinsohn; Eran Pichersky; Natalia Dudareva Journal: Plant J Date: 2013-05-16 Impact factor: 6.417
Authors: Joost Lücker; Mazen K El Tamer; Wilfried Schwab; Francel W A Verstappen; Linus H W van der Plas; Harro J Bouwmeester; Harrie A Verhoeven Journal: Eur J Biochem Date: 2002-07