Literature DB >> 26530685

Purification and Comparative Assay of Human Mitochondrial Single-Stranded DNA-Binding Protein.

Grzegorz L Ciesielski1, Fernando A Rosado-Ruiz2, Laurie S Kaguni3,4.   

Abstract

The mitochondrial single-stranded DNA-binding protein (mtSSB) coordinates the function of replisome components at the mitochondrial replication fork. In recent years, it has been demonstrated that mtSSB stimulates the activities of DNA polymerase γ (Pol γ) and mitochondrial DNA (mtDNA) helicase in a concentration-dependent manner. Here we present a new approach to purify the human mtSSB and our standard assays to evaluate its biochemical properties, including a Gel Mobility Shift Assay (GMSA) to assess single-stranded DNA (ssDNA) binding activity, and an assay to assess SSB stimulation of Pol γ activity.

Entities:  

Keywords:  DNA polymerase γ; Human; Mitochondrial DNA replication; Mitochondrial single-stranded DNA-binding protein

Mesh：

Substances：

Year:  2016        PMID： 26530685      PMCID： PMC4703105          DOI： 10.1007/978-1-4939-3040-1_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

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5.  Functional mitral regurgitation: a link to pulmonary hypertension in heart failure with preserved ejection fraction.

Authors:  Sylvestre Maréchaux; Dan Valentin Neicu; Sophie Braun; Marjorie Richardson; Pascal Delsart; Nadia Bouabdallaoui; Carlo Banfi; Corinne Gautier; Pierre Graux; Philippe Asseman; Philippe Pibarot; Thierry H Le Jemtel; Pierre Vladimir Ennezat
Journal:  J Card Fail       Date:  2011-07-23       Impact factor: 5.712

6.  Functional interactions of mitochondrial DNA polymerase and single-stranded DNA-binding protein. Template-primer DNA binding and initiation and elongation of DNA strand synthesis.

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Journal:  J Biol Chem       Date:  1999-05-21       Impact factor: 5.157

7.  TWINKLE Has 5' -> 3' DNA helicase activity and is specifically stimulated by mitochondrial single-stranded DNA-binding protein.

Authors:  Jenny A Korhonen; Martina Gaspari; Maria Falkenberg
Journal:  J Biol Chem       Date:  2003-09-15       Impact factor: 5.157

8.  Functional roles of the N- and C-terminal regions of the human mitochondrial single-stranded DNA-binding protein.

Authors:  Marcos T Oliveira; Laurie S Kaguni
Journal:  PLoS One       Date:  2010-10-28       Impact factor: 3.240

9.  Single-stranded-DNA-binding proteins from human mitochondria and Escherichia coli have analogous physicochemical properties.

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Journal:  Eur J Biochem       Date:  1994-04-01

Review 10.  Replicating animal mitochondrial DNA.

Authors:  Emily A McKinney; Marcos T Oliveira
Journal:  Genet Mol Biol       Date:  2013-08-30       Impact factor: 1.771
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Journal:  J Clin Invest       Date:  2020-01-02       Impact factor: 14.808

2.  Decrease of MtDNA copy number affects mitochondrial function and involves in the pathological consequences of ischaemic stroke.

Authors:  Zhaojing Zhang; Dongzhi Yang; Baixue Zhou; Yingying Luan; Qihui Yao; Yang Liu; Shangdong Yang; Jing Jia; Yan Xu; Xiaoshuai Bie; Yuanli Wang; Zhihao Li; Aifan Li; Hong Zheng; Ying He
Journal:  J Cell Mol Med       Date:  2022-07-05       Impact factor: 5.295

Review 3.  Nuclear genes involved in mitochondrial diseases caused by instability of mitochondrial DNA.

Authors:  Joanna Rusecka; Magdalena Kaliszewska; Ewa Bartnik; Katarzyna Tońska
Journal:  J Appl Genet       Date:  2018-01-17       Impact factor: 3.240
  3 in total

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