Hung-Chih Chen1,2, Cheng-Mei Yang1,2, Jiin-Tsuey Cheng3, Kuo-Wang Tsai4, Ting-Ying Fu5, Huei-Han Liou4, Hui-Hwa Tseng5, Jang-Hwa Lee5, Guan-Cheng Li4,6, Jyh-Seng Wang5,7, Yu-Yi Hou8,9, Ta-Jung Weng4,6, Luo-Ping Ger4,6. 1. Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 2. Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan. 3. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan. 4. Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 5. Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 6. Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan. 7. School of Medicine, National Yang-Ming University, Taipei, Taiwan. 8. Department of Otorhinolaryngology-Head & Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 9. Department of Nursing, Yuh-Ing Junior College of Health Care and Management, Kaohsiung, Taiwan.
Abstract
BACKGROUNDS: Oral cancer is the 4th leading cause of cancer death for males and the top cancer in young adult males in Taiwan. Tongue squamous cell carcinoma (TSCC) is a common oral cancer and generally associated with poor prognosis. Global DNA hypomethylation at the 5 position of cytosine (5mC) is a well-known epigenetic feature of cancer. Therefore, the purpose of this study was to investigate the relationship of the global 5mC content with the tumorigenesis and prognosis of patients with TSCC. METHODS: The levels of global 5mC were evaluated by immunohistochemistry using tissue microarray slides of 248 surgically resected TSCC and 202 corresponding tumor adjacent normal (TAN) tissues. RESULTS: We found that the level of 5mC in TSCC (P < 0.001) was significantly decreased as compared to TAN. Among TSCC tissues, decreased levels of 5mC were associated with female gender (P = 0.036). In addition, the global hypomethylation was associated with the poor disease-specific survival in TSCC patients (adjusted hazard ratio: 1.55, P = 0.043), especially for patients in older age group (> 50 years, P = 0.013), with moderate or poor cell differentiation (P = 0.044), early stage of disease (I-II, P = 0.046), small tumor size (T1-T2, P = 0.005), without lymph node involvement (P = 0.041), and ever received postoperative radiotherapy (P = 0.009). CONCLUSIONS: Global hypomethylation was an independent biomarker for the development and poor prognosis of TSCC.
BACKGROUNDS: Oral cancer is the 4th leading cause of cancer death for males and the top cancer in young adult males in Taiwan. Tongue squamous cell carcinoma (TSCC) is a common oral cancer and generally associated with poor prognosis. Global DNA hypomethylation at the 5 position of cytosine (5mC) is a well-known epigenetic feature of cancer. Therefore, the purpose of this study was to investigate the relationship of the global 5mC content with the tumorigenesis and prognosis of patients with TSCC. METHODS: The levels of global 5mC were evaluated by immunohistochemistry using tissue microarray slides of 248 surgically resected TSCC and 202 corresponding tumor adjacent normal (TAN) tissues. RESULTS: We found that the level of 5mC in TSCC (P < 0.001) was significantly decreased as compared to TAN. Among TSCC tissues, decreased levels of 5mC were associated with female gender (P = 0.036). In addition, the global hypomethylation was associated with the poor disease-specific survival in TSCC patients (adjusted hazard ratio: 1.55, P = 0.043), especially for patients in older age group (> 50 years, P = 0.013), with moderate or poor cell differentiation (P = 0.044), early stage of disease (I-II, P = 0.046), small tumor size (T1-T2, P = 0.005), without lymph node involvement (P = 0.041), and ever received postoperative radiotherapy (P = 0.009). CONCLUSIONS: Global hypomethylation was an independent biomarker for the development and poor prognosis of TSCC.
Authors: Marta Tagliabue; Pietro Belloni; Rita De Berardinis; Sara Gandini; Francesco Chu; Stefano Zorzi; Caterina Fumagalli; Luigi Santoro; Susanna Chiocca; Mohssen Ansarin Journal: Cancer Med Date: 2021-03-24 Impact factor: 4.452
Authors: Tine Maj Storebjerg; Siri H Strand; Søren Høyer; Anne-Sofie Lynnerup; Michael Borre; Torben F Ørntoft; Karina D Sørensen Journal: Clin Epigenetics Date: 2018-08-07 Impact factor: 6.551