Literature DB >> 26525483

Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis.

Shingo Saito1, Kazuki Hirose, Maho Tsuchida, Koji Wakui, Keitaro Yoshimoto, Yoshitaka Nishiyama, Masami Shibukawa.   

Abstract

We present a polymer-enhanced capillary transient isotachophoresis (PectI) selection methodology for acquisition of high-affinity (kinetically inert) DNA aptamers capable of recognizing distinct microbial cell surfaces, which requires only a single electrophoretic separation between particles (free cells and cells bound with aptamers) and molecules (unbound or dissociated DNA) in free solution.

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Year:  2015        PMID： 26525483     DOI： 10.1039/c5cc07268a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  5 in total

1.  Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM.

Authors:  Junko Enomoto; Tatsuto Kageyama; Tatsuya Osaki; Flavia Bonalumi; Francesca Marchese; Alfonso Gautieri; Elena Bianchi; Gabriele Dubini; Chiara Arrigoni; Matteo Moretti; Junji Fukuda
Journal:  Sci Rep       Date:  2017-03-07       Impact factor: 4.379

2.  Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX.

Authors:  Koji Wakui; Toru Yoshitomi; Akane Yamaguchi; Maho Tsuchida; Shingo Saito; Masami Shibukawa; Hitoshi Furusho; Keitaro Yoshimoto
Journal:  Mol Ther Nucleic Acids       Date:  2019-03-22

3.  Binding and Structural Properties of DNA Aptamers with VEGF-A-Mimic Activity.

Authors:  Toru Yoshitomi; Misako Hayashi; Takumi Oguro; Keiko Kimura; Fumiya Wayama; Hitoshi Furusho; Keitaro Yoshimoto
Journal:  Mol Ther Nucleic Acids       Date:  2020-01-14       Impact factor: 8.886

4.  Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO).

Authors:  A-Ru Kim; Yeongmi Choi; Sang-Heon Kim; Hyun-Seok Moon; Jae-Ho Ko; Moon-Young Yoon
Journal:  Biosensors (Basel)       Date:  2020-10-14

5.  DNA Nanostructures for Targeted Antimicrobial Delivery.

Authors:  Ioanna Mela; Pedro P Vallejo-Ramirez; Stanislaw Makarchuk; Graham Christie; David Bailey; Robert M Henderson; Hiroshi Sugiyama; Masayuki Endo; Clemens F Kaminski
Journal:  Angew Chem Int Ed Engl       Date:  2020-05-20       Impact factor: 16.823
  5 in total

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