Teresa Cimmino1, Abiola Olumuyiwa Olaitan1, Jean-Marc Rolain1,2. 1. a URMITE UM 63 CNRS 7278 IRD 198 INSERM U1905, Méditerranée Infection, Facultés de Médecine et de Pharmacie , Aix-Marseille Université , Marseille Cedex 05 , France. 2. b Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie , Centre Hospitalo-Universitaire Timone, Méditerranée Infection , Marseille Cedex 05 , France.
Abstract
UNLABELLED: We characterize and decipher the resistome and the virulence factors of Shewanella algae MARS 14, a multidrug-resistant clinical strain using the whole genome sequencing (WGS) strategy. The bacteria were isolated from the bronchoalveolar lavage of a hospitalized patient in the Timone Hospital in Marseille, France who developed pneumonia after plunging into the Mediterranean Sea. RESULTS: The genome size of S. algae MARS 14 was 5,005,710 bp with 52.8% guanine cytosine content. The resistome includes members of class C and D beta-lactamases and numerous multidrug-efflux pumps. We also found the presence of several hemolysins genes, a complete flagellum system gene cluster and genes responsible for biofilm formation. Moreover, we reported for the first time in a clinical strain of Shewanella spp. the presence of a bacteriocin (marinocin). CONCLUSION: The WGS analysis of this pathogen provides insight into its virulence factors and resistance to antibiotics.
UNLABELLED: We characterize and decipher the resistome and the virulence factors of Shewanella algae MARS 14, a multidrug-resistant clinical strain using the whole genome sequencing (WGS) strategy. The bacteria were isolated from the bronchoalveolar lavage of a hospitalized patient in the Timone Hospital in Marseille, France who developed pneumonia after plunging into the Mediterranean Sea. RESULTS: The genome size of S. algae MARS 14 was 5,005,710 bp with 52.8% guanine cytosine content. The resistome includes members of class C and D beta-lactamases and numerous multidrug-efflux pumps. We also found the presence of several hemolysins genes, a complete flagellum system gene cluster and genes responsible for biofilm formation. Moreover, we reported for the first time in a clinical strain of Shewanella spp. the presence of a bacteriocin (marinocin). CONCLUSION: The WGS analysis of this pathogen provides insight into its virulence factors and resistance to antibiotics.
Authors: Pedro Seoane; Silvana T Tapia-Paniagua; Rocío Bautista; Elena Alcaide; Consuelo Esteve; Eduardo Martínez-Manzanares; M Carmen Balebona; M Gonzalo Claros; Miguel A Moriñigo Journal: PeerJ Date: 2019-02-28 Impact factor: 2.984