Alice Abreu Ramos1, Maria Prata-Sena2, Bruno Castro-Carvalho2, Tida Dethoup3, Suradet Buttachon2, Anake Kijjoa2, Eduardo Rocha4. 1. Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal. 2. Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal. 3. Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand. 4. Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal. Electronic address: erocha@icbas.up.pt.
Abstract
OBJECTIVE: To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013 (E1), Neosartorya paulistensis KUFC 7897 (E2), Neosartorya siamensis KUFA 0017 (E4) and Talaromyces trachyspermus KUFC 0021 (E3) on a panel of seven human cancer cell lines. METHODS: Effects on cell proliferation, induction of DNA damage and cell death were assessed by MTT and clonogenic assays, comet assay and nuclear condensation assay, respectively. RESULTS: The proliferation of HepG2, HCT116 and A375 cells decreased after incubation with the extracts E2 and E4. The anti-proliferative effect was confirmed by morphologic alterations and by clonogenic assay. Both extracts also induced cell death in HepG2 and HCT116 cells. Doxorubicin was used as a positive control and showed in vitro anticancer activity. CONCLUSIONS: This study demonstrated, for the first time, that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2, HCT16 and A375 cells. The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.
OBJECTIVE: To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013 (E1), Neosartorya paulistensis KUFC 7897 (E2), Neosartorya siamensis KUFA 0017 (E4) and Talaromyces trachyspermus KUFC 0021 (E3) on a panel of seven humancancer cell lines. METHODS: Effects on cell proliferation, induction of DNA damage and cell death were assessed by MTT and clonogenic assays, comet assay and nuclear condensation assay, respectively. RESULTS: The proliferation of HepG2, HCT116 and A375 cells decreased after incubation with the extracts E2 and E4. The anti-proliferative effect was confirmed by morphologic alterations and by clonogenic assay. Both extracts also induced cell death in HepG2 and HCT116 cells. Doxorubicin was used as a positive control and showed in vitro anticancer activity. CONCLUSIONS: This study demonstrated, for the first time, that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2, HCT16 and A375 cells. The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.
Authors: Bruno Castro-Carvalho; Alice A Ramos; Maria Prata-Sena; Fernanda Malhão; Márcia Moreira; Daniela Gargiulo; Tida Dethoup; Suradet Buttachon; Anake Kijjoa; Eduardo Rocha Journal: Pharmacognosy Res Date: 2017-12
Authors: Suradet Buttachon; Alice A Ramos; Ângela Inácio; Tida Dethoup; Luís Gales; Michael Lee; Paulo M Costa; Artur M S Silva; Nazim Sekeroglu; Eduardo Rocha; Madalena M M Pinto; José A Pereira; Anake Kijjoa Journal: Mar Drugs Date: 2018-04-06 Impact factor: 5.118