Isabel Pinilla1, Laura Fernández-Sánchez2, Francisco J Segura3, Ana Isabel Sánchez-Cano4, José Manuel Tamarit5, Lorena Fuentes-Broto6, Janis T Eells7, Pedro Lax2, Nicolás Cuenca2. 1. Department of Ophthalmology, Lozano Blesa University Hospital, Zaragoza, Spain; Aragon Institute for Health Research (IIS Aragon), Zaragoza, Spain; Department of Surgery, School of Medicine, Zaragoza University, Spain. Electronic address: isabel.pinilla@telefonica.net. 2. Department of Physiology Genetics and Microbiology, Alicante University, San Vicente del Raspeig, Alicante, Spain. 3. Aragon Institute for Health Research (IIS Aragon), Zaragoza, Spain; Department of Surgery, School of Medicine, Zaragoza University, Spain. 4. Aragon Institute for Health Research (IIS Aragon), Zaragoza, Spain; Department of Applied Physics, Zaragoza University, Spain. 5. Bloss Group Company, Spain and Heidelberg Engineering Gmbh, Germany. 6. Aragon Institute for Health Research (IIS Aragon), Zaragoza, Spain; Department of Physiology, Zaragoza University, Spain. 7. Department of Biomedical Sciences, University of Wisconsin-Milwaukee, USA.
Abstract
PURPOSE: To characterize the relationship between fundus autofluorescence (FAF), Optical Coherence Tomography (OCT) and immunohistochemistry (IHC) over the course of chronic retinal degeneration in the P23H rat. METHODS: Homozygous albino P23H rats, Sprague-Dawley (SD) rats as controls and pigmented Long Evans (LE) rats were used. A Spectralis HRA OCT system was used for scanning laser ophthalmoscopy (SLO) imaging OCT and angiography. To determine FAF, fluorescence was excited using diode laser at 488 nm. A fast retina map OCT was performed using the optic nerve as a landmark. IHC was performed to correlate with the findings of OCT and FAF changes. RESULTS: During the course of retinal degeneration, the FAF pattern evolved from some spotting at 2 months old to a mosaic of hyperfluorescent dots in rats 6 months and older. Retinal thicknesses progressively diminished over the course of the disease. At later stages of degeneration, OCT documented changes in the retinal layers, however, IHC better identified the cell loss and remodeling changes. Angiography revealed attenuation of the retinal vascular plexus with time. CONCLUSION: We provide for the first time a detailed long-term analysis of the course of retinal degeneration in P23H rats using a combination of SLO and OCT imaging, angiography, FAF and IHC. Although, the application of noninvasive methods enables longitudinal studies and will decrease the number of animals needed for a study, IHC is still an essential tool to identify retinal changes at the cellular level.
PURPOSE: To characterize the relationship between fundus autofluorescence (FAF), Optical Coherence Tomography (OCT) and immunohistochemistry (IHC) over the course of chronic retinal degeneration in the P23Hrat. METHODS: Homozygous albino P23Hrats, Sprague-Dawley (SD) rats as controls and pigmented Long Evans (LE) rats were used. A Spectralis HRA OCT system was used for scanning laser ophthalmoscopy (SLO) imaging OCT and angiography. To determine FAF, fluorescence was excited using diode laser at 488 nm. A fast retina map OCT was performed using the optic nerve as a landmark. IHC was performed to correlate with the findings of OCT and FAF changes. RESULTS: During the course of retinal degeneration, the FAF pattern evolved from some spotting at 2 months old to a mosaic of hyperfluorescent dots in rats 6 months and older. Retinal thicknesses progressively diminished over the course of the disease. At later stages of degeneration, OCT documented changes in the retinal layers, however, IHC better identified the cell loss and remodeling changes. Angiography revealed attenuation of the retinal vascular plexus with time. CONCLUSION: We provide for the first time a detailed long-term analysis of the course of retinal degeneration in P23Hrats using a combination of SLO and OCT imaging, angiography, FAF and IHC. Although, the application of noninvasive methods enables longitudinal studies and will decrease the number of animals needed for a study, IHC is still an essential tool to identify retinal changes at the cellular level.
Authors: Matthew M LaVail; Shimpei Nishikawa; Roy H Steinberg; Muna I Naash; Jacque L Duncan; Nikolaus Trautmann; Michael T Matthes; Douglas Yasumura; Cathy Lau-Villacorta; Jeannie Chen; Ward M Peterson; Haidong Yang; John G Flannery Journal: Exp Eye Res Date: 2017-11-06 Impact factor: 3.467
Authors: Laura Fernández-Sánchez; Irene Bravo-Osuna; Pedro Lax; Alicia Arranz-Romera; Victoria Maneu; Sergio Esteban-Pérez; Isabel Pinilla; María Del Mar Puebla-González; Rocío Herrero-Vanrell; Nicolás Cuenca Journal: PLoS One Date: 2017-05-25 Impact factor: 3.240
Authors: Johnny Di Pierdomenico; Diego García-Ayuso; Isabel Pinilla; Nicolás Cuenca; Manuel Vidal-Sanz; Marta Agudo-Barriuso; María P Villegas-Pérez Journal: Front Neuroanat Date: 2017-03-06 Impact factor: 3.856
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Authors: Laura Fernández-Sánchez; Pedro Lax; Laura Campello; Isabel Pinilla; Nicolás Cuenca Journal: Front Cell Neurosci Date: 2015-12-22 Impact factor: 5.505
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