Literature DB >> 26519312

In Vitro Biochemical Characterization of Cytokinesis Actin-Binding Proteins.

Dennis Zimmermann1, Alisha N Morganthaler1, David R Kovar2,3, Cristian Suarez1.   

Abstract

Characterizing the biochemical and biophysical properties of purified proteins is critical to understand the underlying molecular mechanisms that facilitate complicated cellular processes such as cytokinesis. Here we outline in vitro assays to investigate the effects of cytokinesis actin-binding proteins on actin filament dynamics and organization. We describe (1) multicolor single-molecule TIRF microscopy actin assembly assays, (2) "bulk" pyrene actin assembly/disassembly assays, and (3) "bulk" sedimentation actin filament binding and bundling assays.

Entities:  

Keywords:  Actin; Biomimetics; Cytokinesis; Micropatterning; Microscopy; Single molecule; TIRF

Mesh：

Substances：

Year:  2016        PMID： 26519312     DOI： 10.1007/978-1-4939-3145-3_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Pseudomonas aeruginosa exoenzyme Y directly bundles actin filaments.

Authors:  Jordan M Mancl; Cristian Suarez; Wenguang G Liang; David R Kovar; Wei-Jen Tang
Journal:  J Biol Chem       Date:  2020-02-04       Impact factor: 5.157

2.  Kinetochore protein Spindly controls microtubule polarity in Drosophila axons.

Authors:  Urko Del Castillo; Hans-Arno J Müller; Vladimir I Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  2020-05-19       Impact factor: 11.205

3.  Mechanoregulated inhibition of formin facilitates contractile actomyosin ring assembly.

Authors:  Dennis Zimmermann; Kaitlin E Homa; Glen M Hocky; Luther W Pollard; Enrique M De La Cruz; Gregory A Voth; Kathleen M Trybus; David R Kovar
Journal:  Nat Commun       Date:  2017-09-26       Impact factor: 14.919

4.  The bacterial virulence factors VopL and VopF nucleate actin from the pointed end.

Authors:  Thomas A Burke; Alyssa J Harker; Roberto Dominguez; David R Kovar
Journal:  J Cell Biol       Date:  2017-03-31       Impact factor: 10.539

5.  Cooperation between tropomyosin and α-actinin inhibits fimbrin association with actin filament networks in fission yeast.

Authors:  Jenna R Christensen; Kaitlin E Homa; Alisha N Morganthaler; Rachel R Brown; Cristian Suarez; Alyssa J Harker; Meghan E O'Connell; David R Kovar
Journal:  Elife       Date:  2019-06-10       Impact factor: 8.140

6.  Competition between Tropomyosin, Fimbrin, and ADF/Cofilin drives their sorting to distinct actin filament networks.

Authors:  Jenna R Christensen; Glen M Hocky; Kaitlin E Homa; Alisha N Morganthaler; Sarah E Hitchcock-DeGregori; Gregory A Voth; David R Kovar
Journal:  Elife       Date:  2017-03-10       Impact factor: 8.140

7.  Chlamydomonas reinhardtii formin FOR1 and profilin PRF1 are optimized for acute rapid actin filament assembly.

Authors:  Jenna R Christensen; Evan W Craig; Michael J Glista; David M Mueller; Yujie Li; Jennifer A Sees; Shengping Huang; Cristian Suarez; Laurens J Mets; David R Kovar; Prachee Avasthi
Journal:  Mol Biol Cell       Date:  2019-10-30       Impact factor: 4.138
  7 in total

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