Bettina M Jensen1, Khem B Adhikari2, Heidi J Schnoor3, Nanna Juel-Berg3, Inge S Fomsgaard2, Lars K Poulsen3. 1. Allergy Clinic, Gentofte Hospital, Copenhagen University Hospital, dept. 22, 1. Floor, Hellerup, 2900, Gentofte, Denmark. bettina.margrethe.jensen@regionh.dk. 2. Department of Agroecology, Aarhus University, Slagelse, Denmark. 3. Allergy Clinic, Gentofte Hospital, Copenhagen University Hospital, dept. 22, 1. Floor, Hellerup, 2900, Gentofte, Denmark.
Abstract
PURPOSE:Benzoxazinoids (BXs) are a group of wholegrain phytochemicals with potential pharmacological properties; however, limited information exists on their absorption, metabolism, and excretion in humans. The aim of this study was to investigate the dose-dependent uptake and excretion of dietary BXs in a healthy population. METHODS: Blood and urine were collected from 19 healthy participants from a crossover study after a washout, a LOW BX diet or HIGH BX diet, and analysed for 12 BXs and 4 phenoxazinone derivatives. RESULTS: We found that the plasma BX level peaked approximately 3 h after food intake, whereas BXs in urine were present even at 36 h after consuming a meal. No phenoxazinone derivatives could be detected in either plasma or urine. The dominant BX metabolite in both plasma and urine was 2-β-D-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-Glc), even though 2-β-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one (DIBOA-Glc) was the major component in the diet. CONCLUSION: The dietary BX treatment correlated well with the plasma and urine levels, illustrating strong dose-dependent BX absorption, which also had a rapid washout, especially from the plasma compartment.
RCT Entities:
PURPOSE:Benzoxazinoids (BXs) are a group of wholegrain phytochemicals with potential pharmacological properties; however, limited information exists on their absorption, metabolism, and excretion in humans. The aim of this study was to investigate the dose-dependent uptake and excretion of dietary BXs in a healthy population. METHODS: Blood and urine were collected from 19 healthy participants from a crossover study after a washout, a LOW BX diet or HIGH BX diet, and analysed for 12 BXs and 4 phenoxazinone derivatives. RESULTS: We found that the plasma BX level peaked approximately 3 h after food intake, whereas BXs in urine were present even at 36 h after consuming a meal. No phenoxazinone derivatives could be detected in either plasma or urine. The dominant BX metabolite in both plasma and urine was 2-β-D-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-Glc), even though 2-β-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one (DIBOA-Glc) was the major component in the diet. CONCLUSION: The dietary BX treatment correlated well with the plasma and urine levels, illustrating strong dose-dependent BX absorption, which also had a rapid washout, especially from the plasma compartment.
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