Sherief El-Shazly1, Ali Dashti2, Leila Vali2, Michael Bolaris3, Ashraf S Ibrahim4. 1. Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University, Kuwait; Division of Adult Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, 1124 West Carson St., St. John's Cardiovascular Research Center, Torrance, CA 90502, USA. 2. Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University, Kuwait. 3. Division of Pediatric Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, Torrance, California, USA. 4. Division of Adult Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-University of California Los Angeles (UCLA) Medical Center, 1124 West Carson St., St. John's Cardiovascular Research Center, Torrance, CA 90502, USA; David Geffen School of Medicine at UCLA, Los Angeles, California, USA. Electronic address: ibrahim@labiomed.org.
Abstract
OBJECTIVES: The aim of this study was to identify the genetic relatedness of multiple drug-resistant (MDR) Acinetobacter baumannii clinical isolates recovered from a hospital in Los Angeles. METHODS: Twenty-one MDR A. baumannii isolates were collected and their antibiotic susceptibilities determined according to Clinical and Laboratory Standards Institute guidelines. Genes coding for antibiotic resistance were identified by PCR, and their identities were confirmed by DNA sequencing. Clonal relationships were studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). RESULTS: MDR consistently correlated with the presence of oxacillinases, mostly in the form of the plasmid-mediated OXA-23 enzyme, which was detected in 12 (57.1%) isolates. GES-type carbapenemases were found in 20 (95.2%) strains, AAC in all 21 (100%) strains, and PER in seven (33.3%) strains, and ISAba1 was detected in 16 (76.2%) isolates. The association between ISAba1 and resistance genes confirms insertion elements as a source of β-lactamase production. Of the 21 clinical isolates, five were found to be related to sequence type 1 (ST1) and 16 to ST2, as analyzed by MLST. PFGE demonstrated that the majority of clinical isolates were highly related (>85%). CONCLUSIONS: This study supports a more complete understanding of genotyping of antibiotic resistance for better assessment of MDR strain transmission.
OBJECTIVES: The aim of this study was to identify the genetic relatedness of multiple drug-resistant (MDR) Acinetobacter baumanniiclinical isolates recovered from a hospital in Los Angeles. METHODS: Twenty-one MDR A. baumannii isolates were collected and their antibiotic susceptibilities determined according to Clinical and Laboratory Standards Institute guidelines. Genes coding for antibiotic resistance were identified by PCR, and their identities were confirmed by DNA sequencing. Clonal relationships were studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). RESULTS: MDR consistently correlated with the presence of oxacillinases, mostly in the form of the plasmid-mediated OXA-23 enzyme, which was detected in 12 (57.1%) isolates. GES-type carbapenemases were found in 20 (95.2%) strains, AAC in all 21 (100%) strains, and PER in seven (33.3%) strains, and ISAba1 was detected in 16 (76.2%) isolates. The association between ISAba1 and resistance genes confirms insertion elements as a source of β-lactamase production. Of the 21 clinical isolates, five were found to be related to sequence type 1 (ST1) and 16 to ST2, as analyzed by MLST. PFGE demonstrated that the majority of clinical isolates were highly related (>85%). CONCLUSIONS: This study supports a more complete understanding of genotyping of antibiotic resistance for better assessment of MDR strain transmission.
Authors: George H Talbot; John Bradley; John E Edwards; David Gilbert; Michael Scheld; John G Bartlett Journal: Clin Infect Dis Date: 2005-01-25 Impact factor: 9.079
Authors: Alicia Coelho; Juan José González-López; Elisenda Miró; Carles Alonso-Tarrés; Beatriz Mirelis; María Nieves Larrosa; Rosa María Bartolomé; Antonia Andreu; Ferran Navarro; James R Johnson; Guillem Prats Journal: Int J Antimicrob Agents Date: 2010-04-13 Impact factor: 5.283
Authors: Jennifer M Adams-Haduch; Ezenwa O Onuoha; Tatiana Bogdanovich; Guo-Bao Tian; Jonas Marschall; Carl M Urban; Brad J Spellberg; Diane Rhee; Diane C Halstead; Anthony W Pasculle; Yohei Doi Journal: J Clin Microbiol Date: 2011-09-14 Impact factor: 5.948
Authors: Rebecca H Sunenshine; Marc-Oliver Wright; Lisa L Maragakis; Anthony D Harris; Xiaoyan Song; Joan Hebden; Sara E Cosgrove; Ashley Anderson; Jennifer Carnell; Daniel B Jernigan; David G Kleinbaum; Trish M Perl; Harold C Standiford; Arjun Srinivasan Journal: Emerg Infect Dis Date: 2007-01 Impact factor: 6.883
Authors: Meredith S Wright; Daniel H Haft; Derek M Harkins; Federico Perez; Kristine M Hujer; Saralee Bajaksouzian; Michael F Benard; Michael R Jacobs; Robert A Bonomo; Mark D Adams Journal: MBio Date: 2014-01-21 Impact factor: 7.867
Authors: Mohammed Sami Alhaddad; Afnan Khalifah AlBarjas; Lolowah Ebraheem Alhammar; Abdullatif Sami Al Rashed; Lorina Ineta Badger-Emeka Journal: Int J Appl Basic Med Res Date: 2018 Jan-Mar