| Literature DB >> 26515694 |
Samuel Schmidt1, Merel J W Adjobo-Hermans1, Rike Wallbrecher1, Wouter P R Verdurmen1,2, Petra H M Bovée-Geurts1, Jenny van Oostrum1, Francesca Milletti3, Thilo Enderle4, Roland Brock5.
Abstract
Transfection of cells with a plasmid encoding for the first ten strands of the GFP protein (GFP1-10) provides the means to detect cytosolic peptide import at low micromolar concentrations. Cytosolic import of the eleventh strand of the GFP protein either by electroporation or by cell-penetrating peptide-mediated import leads to formation of the full-length GFP protein and fluorescence. An increase in sensitivity is achieved through structural modifications of the peptide and the expression of GFP1-10 as a fusion protein with mCherry.Keywords: cell uptake; cell-penetrating peptides; drug delivery; fragment complementation assays; lipid membranes
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Year: 2015 PMID： 26515694 DOI： 10.1002/anie.201505913
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336