Literature DB >> 2651467

Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography.

E P Kroeff1, R A Owens, E L Campbell, R D Johnson, H I Marks.   

Abstract

A process based on reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed for the purification of biosynthetic human insulin (BHI). The RP-HPLC procedure has been successfully integrated into the multimodal chromatographic production process used to purify kilogram quantities of BHI. Axial compression column technology was used in the scale-up process. The RP-HPLC procedure yields an insulin product having high chemical purity and full biological activity.

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Year:  1989        PMID： 2651467     DOI： 10.1016/s0021-9673(00)94274-2

Source DB:  PubMed          Journal:  J Chromatogr


  3 in total

1.  Evaluating the conformation of recombinant domain I of β(2)-glycoprotein I and its interaction with human monoclonal antibodies.

Authors:  Charis Pericleous; Jennifer Miles; Diego Esposito; Acely Garza-Garcia; Paul C Driscoll; Anastasia Lambrianides; David Latchman; David Isenberg; Anisur Rahman; Yiannis Ioannou; Ian Giles
Journal:  Mol Immunol       Date:  2011-09-06       Impact factor: 4.407

2.  Conversion of mammalian cell culture media waste to microbial fermentation feed efficiently supports production of recombinant protein by Escherichia coli.

Authors:  Ciara D Lynch; David J O'Connell
Journal:  PLoS One       Date:  2022-05-04       Impact factor: 3.240

Review 3.  Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies.

Authors:  Yin Yin Siew; Wei Zhang
Journal:  Bioresour Bioprocess       Date:  2021-07-27
  3 in total

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