| Literature DB >> 2651408 |
C B Russell1, D S Thaler, F W Dahlquist.
Abstract
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.Entities:
Mesh:
Substances:
Year: 1989 PMID: 2651408 PMCID: PMC209941 DOI: 10.1128/jb.171.5.2609-2613.1989
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490