Paolo Rebulla1, Simonetta Pupella2, Michele Santodirocco3, Noemi Greppi1, Ida Villanova4, Marina Buzzi5, Nicola De Fazio6, Giuliano Grazzini2. 1. Blood Transfusion Centre, Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. 2. Italian National Blood Centre, National Institute of Health, Rome, Italy. 3. Puglia Cord Blood Bank, IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy. 4. Pescara Cord Blood Bank, UOSD Tissue and Biobank, Department of Haematology, Transfusion Medicine and Biotechnologies, Ospedale Civile dello Spirito Santo, Pescara, Italy. 5. Emilia Romagna Cord Blood Bank, Immunohaematology and Transfusion Medicine, Policlinico S. Orsola-Malpighi, Bologna, Italy. 6. Organ and Tissue Transplant Unit, Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Abstract
BACKGROUND: In addition to a largely prevalent use for bleeding prophylaxis, platelet concentrates from adult blood have also been used for many years to prepare platelet gels for the repair of topical skin ulcers. Platelet gel can be obtained by activation of fresh, cryopreserved, autologous or allogeneic platelet concentrates with calcium gluconate, thrombin and/or batroxobin. The high content of tissue regenerative factors in cord blood platelets and the widespread availability of allogeneic cord blood units generously donated for haematopoietic transplant but unsuitable for this use solely because of low haematopoietic stem cell content prompted us to develop a national programme to standardise the production of allogeneic cryopreserved cord blood platelet concentrates (CBPC) suitable for later preparation of clinical-grade cord blood platelet gel. MATERIALS AND METHODS: Cord blood units collected at public banks with total nucleated cell counts <1.5×10(9), platelet count >150×10(9)/L and volume >50 mL, underwent soft centrifugation within 48 hours of collection. Platelet-rich plasma was centrifuged at high speed to obtain a CBPC with target platelet concentration of 800-1,200×10(9)/L, which was cryopreserved, without cryoprotectant, below -40 °C. RESULTS: During 14 months, 13 banks produced 1,080 CBPC with mean (± standard deviation) volume of 11.4±4.4 mL and platelet concentration of 1,003±229×10(9)/L. Total platelet count per CBPC was 11.3±4.9×10(9). Platelet recovery from cord blood was 47.7±17.8%. About one-third of cord blood units donated for haematopoietic transplant could meet the requirements for preparation of CBPC. The cost of preparation was € 160.92/CBPC. About 2 hours were needed for one technician to prepare four CBPC. DISCUSSION: This study yielded valuable scientific and operational information regarding the development of clinical trials using allogeneic CBPC.
BACKGROUND: In addition to a largely prevalent use for bleeding prophylaxis, platelet concentrates from adult blood have also been used for many years to prepare platelet gels for the repair of topical skin ulcers. Platelet gel can be obtained by activation of fresh, cryopreserved, autologous or allogeneic platelet concentrates with calcium gluconate, thrombin and/or batroxobin. The high content of tissue regenerative factors in cord blood platelets and the widespread availability of allogeneic cord blood units generously donated for haematopoietic transplant but unsuitable for this use solely because of low haematopoietic stem cell content prompted us to develop a national programme to standardise the production of allogeneic cryopreserved cord blood platelet concentrates (CBPC) suitable for later preparation of clinical-grade cord blood platelet gel. MATERIALS AND METHODS: Cord blood units collected at public banks with total nucleated cell counts <1.5×10(9), platelet count >150×10(9)/L and volume >50 mL, underwent soft centrifugation within 48 hours of collection. Platelet-rich plasma was centrifuged at high speed to obtain a CBPC with target platelet concentration of 800-1,200×10(9)/L, which was cryopreserved, without cryoprotectant, below -40 °C. RESULTS: During 14 months, 13 banks produced 1,080 CBPC with mean (± standard deviation) volume of 11.4±4.4 mL and platelet concentration of 1,003±229×10(9)/L. Total platelet count per CBPC was 11.3±4.9×10(9). Platelet recovery from cord blood was 47.7±17.8%. About one-third of cord blood units donated for haematopoietic transplant could meet the requirements for preparation of CBPC. The cost of preparation was € 160.92/CBPC. About 2 hours were needed for one technician to prepare four CBPC. DISCUSSION: This study yielded valuable scientific and operational information regarding the development of clinical trials using allogeneic CBPC.
Authors: Eduardo Anitua; José J Aguirre; Jaime Algorta; Eduardo Ayerdi; Ana I Cabezas; Gorka Orive; Isabel Andia Journal: J Biomed Mater Res B Appl Biomater Date: 2008-02 Impact factor: 3.368
Authors: Andrea Piccin; Angela M Di Pierro; Lucia Canzian; Marco Primerano; Daisy Corvetta; Giovanni Negri; Guido Mazzoleni; Günther Gastl; Michael Steurer; Ivo Gentilini; Klaus Eisendle; Fabrizio Fontanella Journal: Blood Transfus Date: 2016-07-25 Impact factor: 3.443