Literature DB >> 26506282

Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept.

V Hinić1, J Ziegler2, C Straub2, D Goldenberger2, R Frei2.   

Abstract

A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).
Copyright © 2015 Elsevier B.V. All rights reserved.

Keywords:  ESBL; Isothermal amplification; Proof of concept; Rapid detection; Urine samples

Mesh：

Substances：

Year:  2015        PMID： 26506282     DOI： 10.1016/j.mimet.2015.10.015

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  7 in total

1.  Evaluation of loop-mediated isothermal amplification for the rapid identification of bacteria and resistance determinants in positive blood cultures.

Authors:  J Rödel; J A Bohnert; S Stoll; L Wassill; B Edel; M Karrasch; B Löffler; W Pfister
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-01-06       Impact factor: 3.267

Review 2.  Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.

Authors:  Godwin Attah Obande; Kirnpal Kaur Banga Singh
Journal:  Infect Drug Resist       Date:  2020-02-12       Impact factor: 4.003

3.  Evaluation of eazyplex® SuperBug CRE Test for Beta-Lactamase Genes Detection in Klebsiella spp. and P. aeruginosa Strains.

Authors:  Alicja Sękowska; Tomasz Bogiel; Eugenia Gospodarek-Komkowska
Journal:  Curr Microbiol       Date:  2019-11-14       Impact factor: 2.188

4.  Rapid and Ultrasensitive Detection of Mutations and Genes Relevant to Antimicrobial Resistance in Bacteria.

Authors:  François Huber; Hans Peter Lang; Daniela Lang; Daniel Wüthrich; Vladimira Hinić; Christoph Gerber; Adrian Egli; Ernst Meyer
Journal:  Glob Chall       Date:  2020-11-30

5.  Risk factors for colonization with multiple species of extended-spectrum beta-lactamase producing Enterobacterales: a case-case-control study.

Authors:  Isabelle Vock; Lisandra Aguilar-Bultet; Adrian Egli; Pranita D Tamma; Sarah Tschudin-Sutter
Journal:  Antimicrob Resist Infect Control       Date:  2021-10-24       Impact factor: 4.887

6.  Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection of Klebsiella pneumoniae and Carbapenemase Genes in Clinical Samples.

Authors:  Aurore C Poirier; Dai Kuang; Bianca S Siedler; Khushboo Borah; Jai W Mehat; Jialin Liu; Cui Tai; Xiaoli Wang; Arnoud H M van Vliet; Wei Ma; David R Jenkins; John Clark; Roberto M La Ragione; Jieming Qu; Johnjoe McFadden
Journal:  Front Mol Biosci       Date:  2022-02-09

7.  The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures.

Authors:  Alicja Sękowska; Tomasz Bogiel
Journal:  Antibiotics (Basel)       Date:  2022-01-21
  7 in total

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