Literature DB >> 26505255

A simple method of image analysis to estimate CAM vascularization by APERIO ImageScope software.

Christian Marinaccio1, Domenico Ribatti.   

Abstract

The chick chorioallantoic membrane (CAM) assay is a well-established method to test the angiogenic stimulation or inhibition induced by molecules and cells administered onto the CAM. The quantification of blood vessels in the CAM assay relies on a semi-manual image analysis approach which can be time consuming when considering large experimental groups. Therefore we present here a simple and fast volumetric method to inspect differences in vascularization between experimental conditions related to the stimulation and inhibition of CAM angiogenesis based on the Positive Pixel Count algorithm embedded in the APERIO ImageScope software.

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Year:  2015        PMID： 26505255     DOI： 10.1387/ijdb.150025dr

Source DB:  PubMed          Journal:  Int J Dev Biol        ISSN： 0214-6282            Impact factor:   2.203


  6 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2022-06-08       Impact factor: 12.779

3.  The EXTrauterine Environment for Neonatal Development Supports Normal Intestinal Maturation and Development.

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Journal:  Cell Mol Gastroenterol Hepatol       Date:  2020-05-28

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Authors:  Johannes Mischinger; Eleonore Fröhlich; Sebastian Mannweiler; Claudia Meindl; Markus Absenger-Novak; Georg C Hutterer; Maximilian Seles; Herbert Augustin; Thomas F Chromecki; Johanna Jesche-Chromecki; Karl Pummer; Richard Zigeuner
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Journal:  Eur J Histochem       Date:  2022-02-17       Impact factor: 3.188

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  6 in total

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