Literature DB >> 26504657

Photophysical characterization of sickle cell disease hemoglobin by multi-photon microscopy.

Genevieve D Vigil1, Scott S Howard1.   

Abstract

The photophysical properties of human sickle cell disease (SCD) Hemoglobin (Hb) is characterized by multi-photon microscopy (MPM). The intrinsic two-photon excited fluorescence (TPEF) signal associated with extracted hemoglobin was investigated and the solidified SCD variant (HbS) was found to demonstrate broad emission peaking around 510 nm when excited at 800 nm. MPM is used to dynamically induce and image HbS gelling by photolysis of deoxygenated HbS. For comparison, photolysis conditions were applied to a healthy variant of human hemoglobin (HbA) and found to remain in solution not forming fibers. The use of this signal to study the mechanism of HbS polymerization associated with the sickling of SCD erythrocytes is discussed.

Entities:  

Keywords:  (020.4180) Multiphoton processes; (190.1900) Diagnostic applications of nonlinear optics

Year:  2015        PMID： 26504657      PMCID： PMC4605066          DOI： 10.1364/BOE.6.004098

Source DB:  PubMed          Journal:  Biomed Opt Express        ISSN： 2156-7085            Impact factor:   3.732


  19 in total

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Authors:  Zakari Y Aliyu; Ashaunta R Tumblin; Gregory J Kato
Journal:  Haematologica       Date:  2006-01       Impact factor: 9.941

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Authors:  G Omar Clay; Chris B Schaffer; David Kleinfeld
Journal:  J Chem Phys       Date:  2007-01-14       Impact factor: 3.488

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Authors:  Peter G Vekilov
Journal:  Br J Haematol       Date:  2007-10       Impact factor: 6.998

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Authors:  Dong Li; Wei Zheng; Wei Zhang; Seng Khoon Teh; Yan Zeng; Yi Luo; Jianan Y Qu
Journal:  Opt Lett       Date:  2011-07-15       Impact factor: 3.776

5.  Kinetics of sickle cell biorheology and implications for painful vasoocclusive crisis.

Authors:  E Du; Monica Diez-Silva; Gregory J Kato; Ming Dao; Subra Suresh
Journal:  Proc Natl Acad Sci U S A       Date:  2015-01-20       Impact factor: 11.205

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Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

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Authors:  J Hofrichter; P D Ross; W A Eaton
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

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Authors:  M Murayama
Journal:  Clin Chem       Date:  1967-07       Impact factor: 8.327

Review 9.  The intracellular polymerization of sickle hemoglobin and its relevance to sickle cell disease.

Authors:  C T Noguchi; A N Schechter
Journal:  Blood       Date:  1981-12       Impact factor: 22.113

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Authors:  David C Rees; Thomas N Williams; Mark T Gladwin
Journal:  Lancet       Date:  2010-12-03       Impact factor: 79.321
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  1 in total

1.  Introduction to the bio-optics: design and application.

Authors:  Tomasz Tkaczyk; Chris Xu
Journal:  Biomed Opt Express       Date:  2015-11-18       Impact factor: 3.732
  1 in total

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