Genome Sequence of Type Strain Bacillus decisifrondis E5HC-32T (DSM 11725T), Isolated from Soil Underlying the Decaying Leaf Litter of a Slash Pine Forest.

Bacillus decisifrondis E5HC-32(T) (DSM 11725(T)) is a Gram-positive, subterminal spherical spore-forming, strictly aerobic bacterium. Here, we report the 5,613,728-bp genome sequence of B. decisifrondis E5HC-32(T), which is the first genome information of this species and will provide useful information for genomic taxonomy and phylogenomics of Bacillus-like bacteria.

GENOME ANNOUNCEMENT

The type strain E5HC-32T was isolated from soil in Australia and was named as Bacillus decisifrondis sp. nov. (1). Its phylogenetically close neighbors are Lysinibacillus xylanilyticus (2) and Lysinibacillus macroides (3) according to their 16S rRNA gene sequence similarity. The best growth of B. decisifrondis E5HC-32T was achieved at 30°C and pH 8.4 (1). Notably, there is no other information about B. decisifrondis outside its taxonomical description information so far. Given no available genomic information of B. decisifrondis, its type strain E5HC-32T was selected as one of the research objects in our “genome sequencing project for genomic taxonomy and phylogenomics of Bacillus-like bacteria.” Here, we present the high-quality draft genome sequence of B. decisifrondis E5HC-32T (DSM 11725T).

The genome sequencing of B. decisifrondis E5HC-32T (DSM 11725T) was performed via the Illumina Hiseq 2500 system. Two DNA libraries with insert sizes of 500 bp were constructed and sequenced. After filtering of the 0.67-Gb raw data, 0.66-Gb of clean data was obtained, providing approximately 100-fold coverage. The reads were assembled via SOAPdenovo software version 2 (4), using a key parameter K setting at 77. Through the data assembly, 78 scaffolds with a total length of 5,613,728 bp were obtained, and the contig N50 was 85767 bp. The average length of the scaffolds was 71,970 bp, and the longest and shortest scaffolds were 469,187  and 591 bp, respectively. A total of 89.95% clean reads were aligned back to the genome, which covered 99.01% f the sequence,

The annotation of the genome was performed using the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP) (http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html) utilizing GeneMark, Glimmer, and tRNAscan-SE tools (5). A total of 5,439 genes were predicted, including 5,057 coding sequences (CDS), 294 pseudogenes, 82 tRNAs, and 6 rRNA genes. There were 3,510 and 2,375 genes assigned to the COG and KEGG databases, respectively. The average DNA G+C content was 36.60%, agreeing with the value 41 ± 1 mol% as determined by the thermal denaturation method (1).

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession no. LIYL00000000. The version described in this paper is version LIYL00000000.1.